Summary
In the continuous cell lines of the LepidopteraTrichoplusia ni (TN-368) andSpodoptera littoralis (HPB-SL-26), ecdysone and 20-hydroxyecdysone cause a series of morphological changes: some cells aggregate, elongate and extend long thin processes. The extent and rapidity of changes are dose dependent, 20-hydroxy-ecdysone being much more active then ecdysone. The continuous cell lines ofS. littoralis (UIV-SL-573) andS. frugiperda (IPLB-SF-21) were much less responsive to the presence of the ecdysteroids.
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References
Best-Belpomme, M, Courgeon AM, Echalier G (1980) Development of a model for the study of ecdysteroid action:Drosophila melanogaster cells establishedin vitro. In: Hoffman JA (ed) Progress in ecdysone research. Elsevier, Amsterdam, pp 379–392
Chen TR (1977) In situ detection of mycoplasma contamination in cell cultures by Fluorescent Hoechst 33258 stain. Exp Cell Res 104: 255–262
Cherbas L, Cherbas P, Savakis C, Demetri G, Manteuffel-Cymborowska M, Yonge CD, Williams CM (1980) Studies of ecdysteroid action on aDrosophila cell line. In: Kurstak E, Maramorosch K, Dubendorfer A (eds) Invertebrate systemsin vitro. Elsevier, Amsterdam, pp 217–228
Cherbas P, Cherbas L, Savakis C, Koehler MMD (1981) Ecdysteroid — responsive genes in aDrosophila cell line. Am Zool 21:743–750
Cherbas P, Savakis C, Cherbas L, Koehler MMD (1982) Steroidcontrolled genes expression in aDrosophila cell line. In: Schmitt FO, Bir SJ, Bloom FE (eds) Molecular genetic neuroscience. Raven Press, New York, pp 277–288
Cohen E, Gilbert LI (1972) Metabolic and hormonal studies on two insect cell lines. J Insect Physiol 18:1061–1076
Cohen E, Lanir N, Englander E (1976) Morphological effects and metabolism of the moulting hormone inAedes aegypti cultured cells. Insect Biochem 6:433–439
Courgeon AM (1972) Action of insect hormones at the cellular level. Morphological changes of a diploid cell line ofDrosophila melanogaster treated with ecdysone and several analoguesin vitro. Exp Cell Res 74:327–336
Hilwig I (1980) Changes in nucleus and surface of Lepidoptera cells cultivatedin vitro after infection with nuclear polyhedrosis viruses (Autographa californica) Acta Biol Acad Sci [Hung] 31:141–147
Hink WF (1970) Established insect cell line from the cabbage looperTrichoplusia ni. Nature 226:466–467
Ireland RC, Berger EM, Sirotkin K, Yund MA, Osterbur D, Fristrom J (1982) Ecdysterone induces the transcription of four heat-shock genes inDrosophila S3 cells and imaginal discs. Dev Biol 93:498–507
Knudson DL, Lescott T, Tinsley TW (1980) Establishment of a continuous cell line ofSpodoptera littoralis. In Vitro 16:369–370
Lynn DE, Oberlander H (1981) The effect of cytoskeletal disrupting agents on the morphological response of a clonedManduca sexta cell line to 20-Hydroxy-ecdysone. Wilhelm Roux's Arch 190:150–155
Lynn DE, Oberlander H (1983) The establishment of cell lines from imaginal wing discs ofSpodoptera frugiperda andPlodia interpunctella. J Insect Physiol 29:591–596
Lynn DE, Miller SG, Oberlander H (1982) Establishment of a cell line from lepidopteran wing imaginal discs: induction of newly synthesized proteins by 20-Hydroxyecdysone. Proc Natl Acad Sci USA 79:2589–2593
Marks EP, Holman GM (1979) Ecdysone action on insect cell lines. In Vitro 15:300–307
McIntosh AH, Rechtoris C (1974) Insect cells: colony formation and cloning in agar medium. In Vitro 10:1–5
Oberlander H, Lynn DE (1982) Morphogenesis in insect tissue culture. Adv Cell Culture 2:237–265
O'Connor JD, Chang ES (1981) Cell lines as a model for the study of metamorphosis. In: Gilbert LI, Frieden E (eds) Metamorphosis. Plenum Press, New York, pp 241–261
Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P (1977) Establishment of two cell lines from the insectSpodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13:213–217
Volkman LE, Summers MD (1975) Nuclear polyhedrosis virus detection: relative capabilities of clones developed fromTrichoplusia ni ovarian cell line TN-368 to serve as indicator cells in a plaque assay. Virology 16:1630–1637
Woods DF, Poodry CA (1983) Cell surface proteins ofDrosophila: I. Changes induced by 20-hydroxyecdysone. Dev Biol 96:23–31
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Kislev, N., Segal, I. & Edelman, M. Ecdysteroids induce morphological changes in continuous cell lines of Lepidoptera. Wilhelm Roux' Archiv 193, 252–256 (1984). https://doi.org/10.1007/BF01260347
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DOI: https://doi.org/10.1007/BF01260347