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A study of amylopectin sulfate and bile damage to the gastric mucosal barrier

An experimental study with canine heidenhain pouches

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Abstract

The protective action against mucosal damage of a sulfated polysaccharide, amylopectin sulfate, was examined in the Heidenhain pouches of 3 dogs. The net fluxes of hydrogen and sodium ion were measured before, during, and after exposure of the mucosa to a 10 mM bile acid solution at pH 2. The potential difference (PD) across gastric mucosa was measured during the experiments. Hydrogen ion back-diffusion from and sodium ion gain by the pouch were significantly increased by bile; there was also a simultaneous fall in PD. However, when the pouches were first treated with a 2% aqueous solution of amylopectin sulfate, the net increase in the flux of H+ and Na+ was significantly less after exposure to bile. The fall in PD was also significantly less. These results suggest that amylopectin sulfate protects gastric mucosa against the damaging effect of bile.

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Authors and Affiliations

  1. University Hospital of Wales, Cardiff, Wales

    Brian J. Calcraft MRCP (Senior Registrar in Gastroenterology), John Rhodes MD, MRCP, Susan Cross BPharm, David Hole MSc & Alan Aubrey MS, FRCS

  2. Medical Research Council Epidemiology Unit, Cardiff, Wales

    Brian J. Calcraft MRCP (Senior Registrar in Gastroenterology), John Rhodes MD, MRCP, Susan Cross BPharm, David Hole MSc & Alan Aubrey MS, FRCS

Authors
  1. Brian J. Calcraft MRCP
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  2. John Rhodes MD, MRCP
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  3. Susan Cross BPharm
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  4. David Hole MSc
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  5. Alan Aubrey MS, FRCS
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Calcraft, B.J., Rhodes, J., Cross, S. et al. A study of amylopectin sulfate and bile damage to the gastric mucosal barrier. Digest Dis Sci 19, 1007–1015 (1974). https://doi.org/10.1007/BF01255782

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