Skip to main content
SpringerLink
Log in

Preparation of an infectious ribonucleic acid derived from mouse brains infected with Israel turkey meningo-encephalitis virus

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

An infectious RNA preparation was obtained from mouse brains infected with the hitherto little known Israel turkey meningo-encephalitis virus isolated from diseased turkeys in 1958. The procedure adopted was based on the use of saturated aqueous phenol and bentonite. The infectiousness of the preparation was demonstrated in vivo and in vitro, and the identity of the virus derived thereof was proved by neutralization and fluorescent antibody techniques. The yields of infectivity was of a low order, approximately 0.001 per cent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Komarov, A., andE. Kalmar: A hitherto undescribed disease — turkey meningo encephalitis. Vet. Rec.72, 257–261 (1960).

    Google Scholar 

  2. Porterfield, J. S.: Israel turkey meningo-encephalitis virus. Vet. Rec.73, 392–394 (1961).

    Google Scholar 

  3. Mussgay, M.: Growth cycle of arboviruses in vertebrate and arthropod cells. Progr. med. Virol.6, 193–267 (1964).

    Google Scholar 

  4. Ada, G. L., andS. G. Anderson: The infectivity of preparations made by the action of phenol on dengue I, dengue II, GD VII and herpes simplex viruses. Aust. J. Sci.21, 259–260 (1959).

    Google Scholar 

  5. Anderson, S. G., andG. L. Ada: Murray Valley encephalitis virus: Preparation of an infective “rihonucleic acid” fraction. Aust. J. exp. Biol. med. Sci.37, 353–364 (1959).

    Google Scholar 

  6. Nielsen, G., undJ. Marquardt: Eine infektiöse Untereinheit von Ribonucleinsäurecharakter aus Gehirnen Gelbfiebervirus-infizierter Mäuse. Arch. ges. Virusforsch.12, 335–345 (1962).

    Google Scholar 

  7. Sokol, F., H. Labikova, andJ. Zemla: Infectious ribonucleic acid from mouse brain infected with tick-borne encephalitis virus. Nature (Lond.)184, 1581 (1959).

    Google Scholar 

  8. Gierer, A., andG. Schramm: Infectivity of ribonucleic acid from tobacco mosaic virus. Nature (Lond.)177, 702–703 (1956).

    Google Scholar 

  9. Fraenkel-Conrat, H., B. Singer, andA. Tsugita: Purification of viral RNA by means of bentonite. Virology14, 54–58 (1961).

    Google Scholar 

  10. Reed, L. J., andH. Muench: A simple method of estimating fifty per cent end-points. Amer. J. Hyg.27, 493–497 (1938).

    Google Scholar 

  11. Holland, J. J., B. H. Hoyer, L. C. McLaren, andJ. T. Syverton: Enteroviral ribonucleic acid. I. Recovery from virus and assimilation by cells. J. exp. Med.112, 821–839 (1960).

    Google Scholar 

  12. Kalnitsky, G., J. P. Hummel, andC. Dierks: Some factors which affect the enzymatic digestion of ribonucleic acid. J. biol. Chem.234, 1512–1516 (1959).

    Google Scholar 

  13. Winnick, T., andR. Goldwasser: Immunological investigation on the origin of myosin of skeletal muscle. Exp. Cell Res.25, 428–436 (1961).

    Google Scholar 

  14. Chadwick, C. S., M. G.McEntegart, and R. C.Nairn: Fluorescent protein tracers: A simple alternative to fluorescein. Lancet, 412–414 (1958).

  15. Smith, C. W., J. D. Marshall, andW. C. Eveland: Use of contrasting fluorescent dye as counterstain in fixed tissue preparations. Proc. Soc. exp. Biol. (N.Y.)102, 179–181 (1959).

    Google Scholar 

  16. Schaffer, F. L.: Physical and chemical properties and infectivity of RNA from animal viruses. Cold Spr. Harb. Symp. quant. Biol.27, 89–99 (1962).

    Google Scholar 

Download references

Author information

Author notes

  1. Y. Nir

    Present address: Israel Institute for Biological Research, Ness-Ziona, Israel

Authors and Affiliations

    Authors
    1. Y. Nir
      View author publications

      You can also search for this author in PubMed Google Scholar

    Rights and permissions

    Reprints and permissions

    About this article

    Cite this article

    Nir, Y. Preparation of an infectious ribonucleic acid derived from mouse brains infected with Israel turkey meningo-encephalitis virus. Archiv f Virusforschung 29, 215–221 (1970). https://doi.org/10.1007/BF01249307

    Download citation

    • Received:

    • Issue Date:

    • DOI: https://doi.org/10.1007/BF01249307

    Keywords

    Search

    Navigation