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Identification of RAPD markers linked to a major blast resistance gene in rice

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Abstract

Rice blast, caused byPyricularia grisea, is a major production constraint in many parts of the world. The Korean rice variety Tongil showed high levels of resistance for about six years when widely planted under highly disease-conducive conditions, before becoming susceptible. Tongil was found to carry a single dominant gene, designatedPi-10t, conferring resistance to isolate 106 of the blast pathogen from the Philippines. We report here the use of bulked segregant RAPD analysis for rapid identification of DNA markers linked toPi-10t. Pooled DNA extracts from five homozygous blast-resistant (RR) and five susceptible (rr) BC3F2 plants, derived from a CO39 × Tongil cross, were analyzed by RFLP using 83 polymorphic probes and by RAPD using 468 random oligomers. We identified two RAPD markers linked to thePi-10t locus: RRF6 (3.8 ± 1.2 cM) and RRH18 (2.9 ± 0.9 cM). Linkage of these markers withPi-10t was verified using an F2 population segregating forPi-10t. The two linked RAPD markers mapped 7 cM apart on chromosome 5. Chromosomal regions surrounding thePi-10t gene were examined with additional RFLP markers to define the segment introgressed from the donor genome.Pi-10t is likely to be a new blast-resistance locus, because no other known resistance gene has been mapped on chromosome 5. These tightly linked RAPD markers could facilitate early selection of thePi-10t locus in rice breeding programmes.

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Authors and Affiliations

  1. Dept. of Microbiology and Biotechnology Center, Faculty of Science, Maharaja Sayajirao University, 390 002, Baroda, India

    Naweed I. Naqvi & Bharat B. Chattoo

  2. Du Pont Agricultural Products, P.O. Box 30, 19714, Newark, DE, USA

    J. Michael Bonman

  3. USDA-ARS, Dept. of Agronomy & Range Science, University of California, 95616, Davis, CA, USA

    David J. Mackill

  4. International Rice Research Institute, P.O. Box 933, 1099, Manila, Philippines

    Rebecca J. Nelson

Authors
  1. Naweed I. Naqvi
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  2. J. Michael Bonman
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  3. David J. Mackill
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  4. Rebecca J. Nelson
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  5. Bharat B. Chattoo
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Naqvi, N.I., Bonman, J.M., Mackill, D.J. et al. Identification of RAPD markers linked to a major blast resistance gene in rice. Mol Breeding 1, 341–348 (1995). https://doi.org/10.1007/BF01248411

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  • DOI: https://doi.org/10.1007/BF01248411

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