Abstract
DNA sequence data were used to examine phylogenetic relationships between six species of economically important Tetranychidae mites:Eotetranychus carpini (Oudemans),E. pruni (Reck),Tetranychus pacificus McGregor,T. mcdanieli McGregor,T. turkestani Ugarov & Nikolski andT. urticae Koch. With primers directed toward conserved elements flanking the target region, the Polymerase Chain Reaction was used to amplify the ITS2 spacer of the ribosomal DNA molecule. The nucleotide sequence of a 300-bp fragment of the ITS2 was determined by direct sequencing and nucleotide divergence used for intra-generic comparison in mites. The resulting phylogenetic tree expressing interspecific relationships in genusTetranychus agrees with morphological data. The study demonstrates the usefulness of the approach in the assessment of the systematics and evolution of the group.
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References
Corpet, F., 1988. Multiple sequence alignment with hierarchical clustering. Nucl. Acids Res., 16: 10881–10889.
Depres, L., Imbert-Establet, D., Combes, C. and Bonhomme, F., 1992. Molecular evidence linking hominid evolution to recent radiation of Schistosomes (Platyhelminths, Trematodes). Mol. Phyl. Evol., in press.
Dilworth, R.J., Chua, K.Y. and Thomas, W.R., 1991. Sequence analysis of cDNA coding for a major house dust mite allergen Der f II. Clin. Exp. Allergy, 21: 25–32.
Grafton-Cardwell, E.E., Eash, J.A. and Granett, J., 1988. Isozyme differentiation ofTetranychus pacificus fromT. urticae andT. turkestani (Acari: Tetranychidae) in laboratory and field populations. J. Econ. Entomol., 81: 770–775.
Gutierrez, J., Helle, W. and Bolland, H.R., 1970. Etude cytogénétique et réflexions phylogénétiques sur la famille des Tetranychidae Donnadieu. Acarologia, XII(4): 732–751.
Gutierrez, J. and Helle, W. 1985. Evolutionary changes in the Tetranychidae. In: W. Helle and M.W. Sabelis (Editors), Spider Mites. Their Biology, Natural Enemies and Control, Vol 1A. Elsevier, Amsterdam, pp. 91–107.
Hillis, D.M., 1987. Molecular versus morphological approaches to systematics. Ann. Rev. Ecol. Syst., 18: 23–42.
Hillis, D.M. and Dixon, M.T., 1991. Ribosomal DNA: molecular evolution and phylogenetic inference. Q. Rev. Biol., 66: 411–453.
Jukes, Y.H. and Cantor, C.R., 1969. Evolution of protein molecules. In: H.N. Munro (Editor), Mammalian Protein Metabolism. Academic Press, New York, pp. 21–32.
Kim, S.S. and Lee, S.C., 1990. Development of acaricidal resistance esterase isozyme ofTetranychus urticae (Acarina: Tetranychidae). Korean J. Appl. Entomol., 29: 170–175.
Miyamoto, M.M. and Cracraft, J., 1992. Phylogenetic analysis of DNA sequences. Oxford University Press, 400 pp.
Mullis, K.B. and Faloona, F.A., 1987. Specific synthesis of DNA in vitro via a polymerasecatalyzed chain reaction. Methods Enzymol., 155: 335–350.
Qu, L.H., Nicoloso, M. and Bachellerie, J.P., 1988. Phylogenetic calibration of the 5′ terminal domain of large rRNA achieved by determining twenty eucaryotic sequences. J. Mol. Evol., 28: 113–124.
Rand, K.N., Moore, T., Sriskantha, A., Spring, K., Tellam, R., Willadsen, P. and Cobon, G.S., 1989. Cloning and expression of a protective antigen from the cattle tickBoophilus microplus. Proc. Natl. Acad. Sci. USA, 86: 9657–9661.
Saiki, R., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B. and Erlich, H.A., 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239: 487–491.
Sogin, M.L. and Gunderson, J.H., 1987. Structural diversity of eukaryotic small subunit ribosomal RNAs: evolutionary implications. Endocytobiology III. Ann. N. Y. Acad. Sci., 503: 125–139.
Sula, J. and Weyda, F., 1983. Esterase polymorphism in several populations of the two-spotted spider mite,Tetranychus urticae Koch. Experientia, 39: 78–79.
Tomaiski, M.D. and Miller, L.K., 1991. Insect paralysis by baculovirus-mediated expression of a mite neurotoxin gene. Nature, 352: 82–85.
Tovey, E.R., Johnson, M.C., Roche, A.L., Cobon, G.S. and Baldo, B.A., 1989. Cloning and sequencing of a cDNA expressing a recombinant house dust mite protein that binds human IgE and corresponds to an important low molecular weight allergen. J. Exp. Med., 170: 1457–1462.
Ward, P.S., Boussy, I.A. and Swincer, D.E., 1982. Electrophoretic detection of enzyme polymorphism and differentiation in three species of spider mites (Tetranychus) (Acari: Tetranychidae). Ann. Entomol. Soc. Am., 75: 595–598.
Yuuki, T., Okumura, Y., Ando, T., Yamakawa, H., Suko, M., Haida, M. and Okudaira, H., 1991. Cloning and expression of cDNA coding for the major house dust mite allergen Der f II inEscherichia coli. Agric. Biol. Chem., 55: 1232–1238.
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Navajas, M., Cotton, D., Kreiter, S. et al. Molecular approach in spider mites (Acari: Tetranychidae): Preliminary data on ribosomal DNA sequences. Exp Appl Acarol 15, 211–218 (1992). https://doi.org/10.1007/BF01246563
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DOI: https://doi.org/10.1007/BF01246563