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Matching localization of vasoactive intestinal polypeptide (VIP) and VIP-receptor at pre- and postsynaptic sites in the mouse visual cortex

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Journal of Neurocytology

Summary

Vibratome sections of the mouse occipital cortex were processed by double label immunohistochemistry to demonstrate the localization of the receptor for vasoactive intestinal polypeptide and the peptide itself. The receptor was found to be distributed in the cytoplasm and major dendrites of numerous cortical cells, mainly pyramidal neurons. Vasoactive intestinal polypeptide, on the other hand, occurred in a population of non-pyramidal neurons and axonal boutons. Image analysis revealed a close spatial association of peptide-containing presynaptic terminals with receptor-containing cells. Ultrastructurally, these connections represented symmetrical axo-somatic and axo-dendritic synapses. Our findings demonstrate a matching histological localization of vasoactive intestinal polypeptide and its receptor in the brain.

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References

  • Besson, J., Sarrieau, A., Vial, M., Marie, J.-C., Rosselin, G. &Rostene, W. (1986) Characterization and autoradiographic distribution of vasoactive intestinal peptide binding sites in the rat central nervous system.Brain Research 398, 329–36.

    Google Scholar 

  • Connor, J. R. &Peters, A. (1984) Vasoactive intestinal polypeptide immunoreactive neurons in rat visual cortex.Neuroscience 12, 1027–44.

    Google Scholar 

  • De Souza, E. B., Seifert, H. &Kuhar, M. J. (1985) Vasoactive intestinal peptide receptor localization in rat forebrain by autoradiography.Neuroscience Letters 56, 113–20.

    Google Scholar 

  • Dietl, M. M., Hof, P. R., Martin, J.-L., Magistretti, P. J. &Palacios, J. M. (1990) Autoradiographic analysis of the distribution of vasoactive intestinal peptide binding sites in the vertebrate central nervous system: a phylogenetic study.Brain Research 520, 14–26.

    Google Scholar 

  • Fuxe, K., HÖkfelt, T., Said, S. I. &Mutt, V. (1977) VIP and the nervous system: immunohistochemical evidence for localization in central and peripheral nerves, particularly intracortical neurons of the cerebral cortex.Neuroscience Letters 5, 241–6.

    Google Scholar 

  • Gallyas, F., Görcs, T. &Me-Rchenthaler, I. (1982) High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry.Journal of Histochemistry and Cytochemistry 30, 183–4.

    Google Scholar 

  • Görcs, T., Léránth, Cs. &MacLusky, N. J. (1986) The use of gold-substituted silver-intensified diaminobenzidine (DAB) and non-intensified DAB for simultaneous electron microscopic immunoperoxidase labelling of tyrosine hydroxylase and glutamic acid decarboxylase immunoreactivity in the rat medial preoptic area.Journal of Histochemistry and Cytochemistry 34, 1439–47.

    Google Scholar 

  • Gozes, I. &Brenneman, D. E. (1989) VIP: molecular biology and neurobiological function.Molecular Neurobiology 201, 201–35.

    Google Scholar 

  • Hajós, F. &Zilles, K. (1988) Quantitative immunohistochemical analysis of VIP-neurons in the rat visual cortex.Histochemistry 90, 139–44.

    Google Scholar 

  • Hajós, F., Zilles, K., Gallatz, K., Schleicher, A., Kaplan, I. &Werner, L. (1988a) Ramification patterns of vasoactive intestinal polypetide (VIP)-cells in the rat primary visual cortex. An immunohistochemical study.Anatomy and Embryology 178, 197–206.

    Google Scholar 

  • Hajós, F., Zilles, K., Schleicher, A. &Kálmán, M. (1988b) Types and spatial distribution of vasoactive intestinal polypeptide (VIP)-containing synapses in the rat visual cortex.Anatomy and Embryology 178, 207–17.

    Google Scholar 

  • Hof, P. R., Dietl, M. M., Charnay, Y., Martin, J.-L., Bouras, C., Palacios, J. M. &Magistretti, P. J. (1991) Vasoactive Intestinal Peptide binding sites and fibers in the brain of the pigeonColumba livia: an autoradiographic and immunohistochemical study.Journal of Comparative Neurology 305, 393–411.

    Google Scholar 

  • Höfer, M., Schröder, H. &Zilles, K. (1991) Darstellung von Rezeptoren für vasoaktives intestinales Polypeptid (VIP) im Cortex cerebri des Menschen mittels Rezeptorantikörpern.Anatomischer Anzeiger 168 (Suppl.) 609–10.

    Google Scholar 

  • Magistretti, P. J. &Morrison, J. H. (1985) VIP-neurons in the neocortex.Trends in Neuroscience 8, 7–8.

    Google Scholar 

  • Martin, J.-L., Rose, K., Hughes, G. J. &Magistretti, P. J. (1986) [mono[125I]iodo-Tyr10, Meto17]-Vasoactive Intestinal Polypeptide. Preparation, characterization, and use for radioimmunoassay and receptor binding.Journal of Biological Chemistry 261, 5320–7.

    Google Scholar 

  • Martin, J.-L., Dietl, M. M., Hof, P. R., Palacios, J. M. &Magistretti, P. J. (1987) Autoradiographic mapping of [mono[125I]iodo-Tyr10, Meto17]-Vasoactive Intestinal peptide binding sites in the rat brain.Neuroscience 23, 539–65.

    Google Scholar 

  • McDonald, J. K., Parnavelas, J. G., Karamanlidis, A. N. &Brecha, N. (1982) The morphology and distribution of peptide containing neurons in adult and developing visual cortex of the rat. II. Vasoactive intestinal polypeptide.Journal of Neuroscience 11, 825–37.

    Google Scholar 

  • Morrison, J. H., Magistretti, P. J., Benoit, R. &Bloom, F. E. (1984) The distribution and morphological characteristics of the intracortical VIP-cell: an immunohistochemical analysis.Brain Research 292, 269–82.

    Google Scholar 

  • Paul, S. &Said, S. I. (1985) Solubilization and physical separation of active high- and low-affinity receptors for vasoactive intestinal peptide (VIP).Regulatory Peptides 3, 52.

    Google Scholar 

  • Peters, A. (1990) The axon terminals of vasoactive intestinal polypeptide (VIP)-containing bipolar cells in rat visual cortex.Journal of Neurocytology 19, 672–85.

    Google Scholar 

  • Peters, A. &Harriman, K. M. (1988) Enigmatic bipolar cell of rat visual cortex.Journal of Comparative Neurology 267, 409–32.

    Google Scholar 

  • Pichon, J., Hirn, M., Muller, J. M., Mangeat, P. &Marvaldi, J. (1983) Anti-cell surface monoclonal antibodies which antagonize the action of VIP in a human adenocarcinoma cell line (HT 29 cells).The EMBO Journal 2, 1017–22.

    Google Scholar 

  • Robberecht, P., Cauvin, A., Gourlet, P. &Christophe, J. (1990) Heterogeneity of VIP receptors.Archives Internationales de Pharmacodynamie et Therapie 303, 51–66.

    Google Scholar 

  • Sims, K. B., Hoffman, D. L., Said, S. I. &Zimmermann, E. A. (1980) Vasoactive intestinal polypeptide (VIP) in mouse and rat brain. An immunocytological study.Brain Research 186, 165–83.

    Google Scholar 

  • Zilles, K., Kálmán, M., Hajós, F. &Schleicher, A. (1991) Developmental gradients of vasoactive intestinal polypeptide (VIP)-containing neurons in the rat visual cortex detected by image analysis.Developmental Brain Research 60, 137–44.

    Google Scholar 

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Authors and Affiliations

  1. 1st Department of Anatomy, Semmelweis University of Medicine, Budapest, Hungary

    A. Csillag

  2. Department of Anatomy and Histology, University of Veterinary Science Budapest, Budapest, Hungary

    F. Hajós

  3. C. & O. Vogt Institute for Brain Research, University of Düsseldorf, Düsseldorf, Germany

    K. Zilles & A. Schleicher

  4. Institute 2 of Anatomy, University of Cologne, Cologne, Germany

    H. Schröder

Authors
  1. A. Csillag
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  2. F. Hajós
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  3. K. Zilles
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  4. A. Schleicher
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  5. H. Schröder
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Csillag, A., Hajós, F., Zilles, K. et al. Matching localization of vasoactive intestinal polypeptide (VIP) and VIP-receptor at pre- and postsynaptic sites in the mouse visual cortex. J Neurocytol 22, 491–497 (1993). https://doi.org/10.1007/BF01181568

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  • DOI: https://doi.org/10.1007/BF01181568

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