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An experimental analysis of interlamellar tight junctions in amphibian and mammalian C.N.S. myelin

  • Published:
Journal of Neurocytology

Summary

The distribution of interlamellar tight junctions was examined in myelin sheaths ofXenopus tadpole optic nerve and rabbit epiretinal tissue fixed with aldehydes, postfixed with osmium ferrocyanide and embedded in a water-soluble medium, Durcupan. Intramyelinic zonulae occludentes were clearly formed by fusion of adjacent intraperipd lines which corresponded to the external leaflets of oligodendrocytes. These occurred in register with other tight junctions present within successive lamellae and appeared as a series of radial lines extending either partially or totally across the thickness of the myelin sheath. This distribution of zonulae occludentes corresponded with that of tight junctional particle strands observed in freeze-fracture replicas.

Analysis of intramyelinic vacuolation induced by hexachlorophene (HCP) intoxication indicated that lamellar splitting was frequently limited by the tight junctions. The intramyelinic zonulae occludentes also restricted the diffusion of colloidal lanthanum which had penetrated the myelin intraperiod gap followingin vivo perineural injection. The results of this study provide evidence favouring a correspondence between interlamellar tight junctions and the ‘radial component’ of myelin described earlier by other investigators. Furthermore, observations of swollen myelin sheaths, resulting from HCP intoxication, suggest that these junctions may play a major role in maintaining myelin sheath integrity and limiting the extent of breakdown during certain pathological conditions.

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Author notes

  1. T. Tabira

    Present address: Department of Neurology, Faculty of Medicine, Kyushu University, 812, Fukuoka City, Japan

Authors and Affiliations

  1. Laboratory of Neuropathology and Neuroanatomical Sciences, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, 20014, Bethesda, MD, USA

    T. Tabira, M. J. Cullen & H. deF. Webster

  2. Department of Anatomy, University of Maryland School of Medicine, 21201, Baltimore, MD, USA

    P. J. Reier

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  1. T. Tabira
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  2. M. J. Cullen
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  3. P. J. Reier
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  4. H. deF. Webster
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Tabira, T., Cullen, M.J., Reier, P.J. et al. An experimental analysis of interlamellar tight junctions in amphibian and mammalian C.N.S. myelin. J Neurocytol 7, 489–503 (1978). https://doi.org/10.1007/BF01173993

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  • DOI: https://doi.org/10.1007/BF01173993

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