Abstract
Interspecific somatic fusion was performed between ‘Seminole’ tangelo (Citrus reticulata Blanco xC. paradisi Macf.) protoplasts isolated from embryogenic callus and rough lemon (C. jambhiri Lush.) mesophyll protoplasts. Eight plants out of ten randomly selected regenerants had 18 chromosomes and the same nuclear rDNA fragment patterns as that of the mesophyll parent. The remaining two plants showed rDNA fragment patterns from both parents and had 36 chromosomes. For the analysis of mitochondrial DNA (mtDNA),rrn26 derived from pea was used to probeBamHI digests of the regenerants. All plants showed mtDNA band patterns identical to that of the callus parent, suggesting that eight plants were cybrids and the remaining two plants were somatic hybrids. In addition to the callus parent band patterns, additional fragments from the mesophyll parent and/or a novel band fragment were revealed in some of the putative cybrids by peaatpA probe after digestion withDraI andPstI. These results suggest the occurrence of mtDNA recombination/rearrangement inCitrus cybrids produced by somatic fusion in this interspecific combination.
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Abbreviations
- mtDNA :
-
Mitochondrial DNA
References
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Grosser JW, Gmitter FG Jr, Tusa N, Reforgiato Recupero G, Cucinotta P (1996) Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion. Plant Cell Rept 15:672–676
Hidaka T, Omura M (1992) Regeneration of somatic hybrid plants obtained by electrical fusion between satsuma mandarin (Citrus unshiu) and rough lemon (C. jambhiri) or yuzu (C. junos). Japan J Breed 47:79–89
Hidaka T, Moriguchi T, Motomura T, Katagi S, Omura M (1995) Development of a new electrode chamber and its efficiency in protoplast fusion inCitrus. Breed Sci 45:237–239
Ichikawa H, Tanno-Suenaga L, Imamura J (1989) Transfer of mitochondria through protoplast fusion. In: Bajaj YPS (ed) Plant protoplasts and genetic engineering II. Biotechnology in agriculture and forestry, vol 9. Springer, Berlin Heidelberg New York, pp 360–375
Ito Y, Omura M, Nesumi H, Yoshida T (1992) Improvement of preparation and observation methods forCitrus chromosomes. Bull Fruit Tree Res Station (Ministry Agric For Fish) 23:57–66
Kao KN (1977) Chromosome behaviour in somatic hybrids of soybean-Nicotiana glauca. Mol Gen Genet 150:225–230
Kobayashi S, Ohgawara T, Ohgawara E, Oiyama I, Ishii S (1988) A somatic hybrid plant obtained by protoplast fusion between navel orange (Citrus sinensis) and satsuma mandarin (C. unshiu). Plant Cell Tissue Organ Cult 14:63–69
Kobayashi S, Ohgawara T, Fujiwara K, Oiyama I (1991) Analysis of cytoplasmic genomes in somatic hybrids between navel orange (Citrus sinensis Osb.) and ‘Murcott’ tangor. Theor Appl Genet 82:6–10
Kumar A, Cocking ED (1987) Protoplast fusion: a novel approach to organelle genetics in higher plants. Am J Bot 74:1289–1303
Moriguchi T, Motomura T, Hidaka T, Akihama T, Omura M (1996) Genotype and parental combination influence efficiency of cybrid induction inCitrus by electrofusion. HortScience 31:275–278
Motomura T, Hidaka T, Moriguchi T, Akihama T, Omura M (1995) Intergeneric somatic hybrids betweenCitrus andAtalantia orSeverinia by electrofusion, and recombination of mitochondrial genomes. Breed Sci 45:309–314
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Ohgawara T, Kobayashi S, Ohgawara E, Uchimiya H, Ishii S (1985) Somatic hybrid plants obtained by protoplast fusion betweenCitrus sinensis andPoncirus trifoliata. Theor Appl Genet 71:1–4
Saito W, Ohgawara T, Shimizu J, Ishii S, Kobayashi S (1993)Citrus cybrid regeneration following cell fusion between nucellar cells and mesophyll cells. Plant Sci 88:195–201
Saito W, Ohgawara T, Shimizu J, Kobayashi S (1994) Somatic hybridization inCitrus using embryogenic cybrid callus. Plant Sci 99:89–95
Takaiwa F, Oono K, Sugiura M (1984) The complete nucleotide sequence of a rice 17s rRNA gene. Nucleic Acids Res 12:5441–5444
Vardi A, Spiegel-Roy P, Galun E (1982) Plant regeneration from citrus protoplasts: variability in methodological requirements among cultivars and species. Theor Appl Genet 62:171–176
Vardi A, Breiman A, Galun E (1987)Citrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles. Theor Appl Genet 75:51–58
Vardi A, Arzee-Gonen P, Frydman-Shani A, Bleichman S, Galun E (1989) Protoplast-fusion-mediated transfer of organelles fromMicrocitrus Citrus and regeneration of novel alloplasmic trees. Theor Appl Genet 78:741–747
Yamamoto M, Kobayashi S (1995) A cybrid plant produced by electrofusion betweenCitrus unshiu (satsuma mandarin) andC. sinensis (sweet orange). Plant Tissue Cult Lett 12:131–137
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Moriguchi, T., Motomura, T., Hidaka, T. et al. Analysis of mitochondrial genomes amongCitrus plants produced by the interspecific somatic fusion of ‘Seminole’ tangelo with rough lemon. Plant Cell Reports 16, 397–400 (1997). https://doi.org/10.1007/BF01146781
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DOI: https://doi.org/10.1007/BF01146781