Abstract
Anin vitro core collection of African coffee germplasm, structured in 32 diploid diversity groups, was established and conserved under slow growth for 3 years (6 subcultures). The initial objective was to store twenty accessions per group, with four replicates per accession. A statistical model was developed to analyse observations of survival rates within each diversity group. The goodness of fit of the model was shown. Survival analysis indicated a broad variability of the accessions in their response to the storage conditions and confirmed the importance of structuring the coffee complex down to the intraspecific level. Intra- and inter-group differences had consequences on the genetic representativity of thein vitro core collection. For practical purposes, conservation was carried on when the intra-group genetic drift was less than 50%.
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Abbreviations
- BA:
-
6-benzyladenine
- CAR:
-
Central African Republic
- CIRAD:
-
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
- FAO:
-
Food and Agriculture Organization
- IBPGR:
-
International Board for Plant Genetic Resources
- IDEFOR-DCC:
-
Institut Des Fôrets - Département Café Cacao
- ORSTOM:
-
Institut français de recherche scientifique pour le développement en coopération
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Communicated by G. Pelletier
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Dussert, S., Chabrillange, N., Anthony, F. et al. Variability in storage response within a coffee (Coffea spp.) core collection under slow growth conditions. Plant Cell Reports 16, 344–348 (1997). https://doi.org/10.1007/BF01088294
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DOI: https://doi.org/10.1007/BF01088294