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Effect of 1,1-dimethylhydrazine on lymphoproliferation and interleukin 2 immunoregulatory function

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Abstract

The studies reported here suggest that the immunomodulatory effects of 1,1-dimethylhydrazine (UDMH) are associated, in part, with interference with interleukin 2 (IL-2) regulatory action. Concanavalin A (Con A)-stimulated DNA synthesis in murine splenocytes was inhibited from 18.6 to 44.1% at sub-toxic concentrations of UDMH (10 to 50 μg/ml) and IL-2-dependent DNA synthesis in CTLL-20 cells was inhibited from 11.3 to 41.58% at subtoxic concentrations of UDMH (10 to 50 μg/ml). In addition, UDMH suppressed phorbol myristic acetate (PMA)-stimulated IL-2 production in EL-4 cells by up to 30% and slightly suppressed IL-2 production by Con A-stimulated murine splenocytes. In all cases, inhibition was evident at sub-toxic UDMH concentrations and was demonstrated to be independent of inactivation of IL-2 or interference with IL-2 absorption. It is suggested that UDMH has the potential to modify immune function through interference with IL-2 production and especially the lymphoproliferative response to IL-2.

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  1. Richard M. Bauer

    Present address: Pandex Corp., 909 Orchard St., 60060, Mundelin, Illinois

Authors and Affiliations

  1. Department of Veterinary Pathobiology, Comprehensive Cancer Care Center, The Ohio State University, 43210, Columbus, Ohio, USA

    Richard M. Bauer, Melinda J. Tarr & Richard G. Olsen

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  1. Richard M. Bauer
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  2. Melinda J. Tarr
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  3. Richard G. Olsen
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Bauer, R.M., Tarr, M.J. & Olsen, R.G. Effect of 1,1-dimethylhydrazine on lymphoproliferation and interleukin 2 immunoregulatory function. Arch. Environ. Contam. Toxicol. 19, 148–153 (1990). https://doi.org/10.1007/BF01059824

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  • DOI: https://doi.org/10.1007/BF01059824

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