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Two calcium currents in the somatic membrane of mollusc neurons

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Abstract

Two new types of calcium channels were discovered during research in ionic currents in the somatic membrane ofHelix pomatia neurons, using an intracellular perfusion technique. Apart from the principal calcium current described in the literature with a holding potential of about −110 mV, an additional calcium current was observed activated at depolarizations of −40 to −80 mV and was not reduced when the cell was perfused with solutions containing fluoride anions. The kinetics of this current were well described in the context of the Hodgkin and Huxley model with a time constant of activation of 6–8 msec and of inactivation of 300–600 msec. It increased in amplitude as the Ca++ rose in the cellular environment but was reduced by extracellular addition of the Ca++ antagonists Co++, Ni++, and Cd++, and the organic blockers nifedipine and verapamil. The association constants of these substances with corresponding channels determined from the maximum of the current-voltage relationship were 2 (Ca++), 3 (Co++), 0.06 (nifedipine), and 0.2 mM (verapamil). The properties detected in this component of calcium conductance are compared with those of calcium channels in other excitatory formations and its possible functional role is discussed.

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Authors
  1. S. L. Mironov
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  2. A. V. Tepikin
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  3. A. V. Grishchenko
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Additional information

A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 17, No. 5, pp. 627–633, September–October, 1985.

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Mironov, S.L., Tepikin, A.V. & Grishchenko, A.V. Two calcium currents in the somatic membrane of mollusc neurons. Neurophysiology 17, 451–455 (1985). https://doi.org/10.1007/BF01052180

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  • DOI: https://doi.org/10.1007/BF01052180

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