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A monoclonal antibody that recognizes both leb and Y (Ley) antigens

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Abstract

A hemagglutinating monoclonal antibody has been obtained from a mouse/mouse hybridoma after immunisation with the leb-active oligosaccharide, lacto-N-difucohexaose I, coupled to edestin. The antibody agglutinated human red cells regardless of Lewis phenotype. Blood group O cells were strongly, agglutinated, and progressively weaker agglutination was observed with A2, B and A2B cells. Blood group A1 and A1B cells were not agglutinated.

By examining the binding of the antibody to glycolipids and oligosaccharides it was shown that the Leb and Y (Ley)-haptens bind to a similar extent. Full binding activity was dependent on the presence of, both fucosyl residues.

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Abbreviations

LND l:

lacto-N-difucohexaose l

IV2Fuc,lll4FucLcOse4 :

LND l-OL, lacto-N-difucohexaitol l

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Brodin, T., Alan Chester, M., Karlsson, KA. et al. A monoclonal antibody that recognizes both leb and Y (Ley) antigens. Glycoconjugate J 4, 399–406 (1987). https://doi.org/10.1007/BF01048373

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