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Comparative studies on the nature of purified cytomembranes of the rabbit parotid gland

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Centrifugation procedures have been evolved for isolating purified samples of rough endoplasmic reticulum, Golgi, zymogen granule and plasmalemmal membranes from homogenates of rabbit parotid gland tissue. The purification process was monitored using morphometry and enzyme and chemical marker assays.

The membrane preparations were analysed by sodium dodecylsuphate (SDS) polyacrylamide gel electrophoresis, quantitative phospholipid thin layer chromatography and by enrichment studies. The results were used to evaluate various possible general models for the behaviour of membranes during the secretory cycle of parotid acinar cells.

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References

  • AMAR-COSTESEC, A., WIBO, M., THINES-SEMPOUX, D., BEAUFAY, H. & BERTHET, J. (1974). Analytical study of microsomes and isolated subcellular membranes from rat liver IV.J. Cell Biol. 62, 717–45.

    Google Scholar 

  • AMES, B. N. (1966).Assay of inorganic phosphate, total phosphate and phosphatases. Methods in Enzymology. (eds. E. F. Neufield and B. Ginsberg) Vol. 8. pp. 115–118. London, New York: Academic Press.

    Google Scholar 

  • AMSTERDAM, A. OHAD, I. & SCHRAMM, M. (1969). Dynamic changes in the ultrastructure of the acinar cell of the rat parotid gland during the secretory cycle.J. Cell Biol. 41, 754–73.

    Google Scholar 

  • BARTLETT, C. R. (1959). Phosphorus assay in column chromatography,J. Biol. Chem. 234, 406–8.

    Google Scholar 

  • BERGELSON, L. D., DYATLOVITSKAYA, E. V., TORKHOVSKAYA, T. I., SOROKINA, I. B. & GORKOVA, N. P. (1970). Phospholipid composition of membranes in the tumour cell.Biochem. biophys. Acta 210, 287–98.

    Google Scholar 

  • BERNFIELD, P. (1955). α-and β-Amylases, In:Methods in Enzymology, Vol. 1 (eds. S. P. Colowick and N. P. Kaplan), pp. 149–159, New York, London: Academic Press.

    Google Scholar 

  • BURTON, K. (1956). A study of the conditions and mechanism of the diphenylamine reaction from the colourimetric estimation of deoxyribonucleic acid.Biochem. J. 62, 315–22.

    Google Scholar 

  • CARO, L. G. & PALADE, G. E. (1964). Protein synthesis, storage, and discharge in the pancreatic exocrine cell: an autoradiographic study.J. Cell Biol. 20, 473–95.

    Google Scholar 

  • CASTLE, J. D., JAMIESON, J. D. & PALADE, G. E. (1972). Radioautographic analysis of the secretion process in the parotid acinar cell of the rabbit.J. Cell Biol. 53, 290–311.

    Google Scholar 

  • CASTLE, J. D. & PALADE, G. E. (1978). Secretion granules of the rabbit parotid gland. Selective removal of secretory contaminants from granule membranes.J. Cell Biol. 76, 323–40.

    Google Scholar 

  • COPE, G. H., PRATTEN, M. K. & WILLIAMS, M. A. (1976). Correlative morphological and biochemical study of the effects of isoprenaline on the organelle and membrane content of the rabbit parotid gland.Histochem. J. 8, 403–18.

    Google Scholar 

  • COPE, G. H. & WILLIAMS, M. A. (1973a). Quantitative analyses of the constituent membranes of parotid acinar cells and of the changes evident after induced exocytosis.Z. Zellforsch. 145, 311–30.

    Google Scholar 

  • COPE, G. H. & WILLIAMS, M. A. (1973b). Exocrine secretion in the parotid gland: A stereological analysis at the electron microscopic level of the zymogen granule content before and after isoprenaline-induced degranulation.J. Anat. (London) 116, 269–84.

    Google Scholar 

  • COPE, G. H. & WILLIAMS, M. A. (1974). Improved preservation of parotid tissue for electron microscopy. A method permitting the collection of valid stereological data.J. Cell Biol. 60, 292–7.

    Google Scholar 

  • COPE, G. H. & WILLIAMS, M. A. (1977). Biochemical and histochemical studies onp-nitrophenyl phosphate and β-glycerophosphate hydrolysis at acid pH by rabbit parotid tissue.Histochem. J. 9, 685–701.

    Google Scholar 

  • DURHAM, J. P., GALANTI, N. & REVIS, N. W. (1975). The purification and characterization of plasma membranes and subcellular distribution of adenylate cyclase in mouse parotid gland.Biochem. biophys. Acta 394, 388–405.

    Google Scholar 

  • FAWCETT, D. W. (1962). Physiologically significant specializations of the cell surface.Circulation 26, 1105–25.

    Google Scholar 

  • FISKE, C. H. & SUBBA ROW, Y. (1925). The colorimetric determination of phosphorus.J. biol. Chem. 66, 375–400.

    Google Scholar 

  • FRANKE, W. W., MORRÉ, D. J., DEUMLING, B., CHEETHAM, R. D., KARTENBECK, J., JARASH, E. D., & ZENTGRAF, H. W. (1971).Z. Naturforsch. 26b, 1031–9.

    Google Scholar 

  • FOLCH, J., LEES, M. & SLOANE-STANLEY, G. H. (1957). A simple method for the isolation and purification of total lipids from animal tissues.J. biol. Chem. 226, 497–509.

    Google Scholar 

  • GLAUMANN, H. & DALLNER, G. (1968). Lipid composition of rough and smooth microsomal membranes in rat liver.J. Lipid Res. 9, 720–9.

    Google Scholar 

  • HOKIN, L. E. (1968). Dynamic aspects of phospholipids during protein secretion.Int. Rev. Cytol. 23, 187–208.

    Google Scholar 

  • JAMIESON, J. E. & PALADE, G. E. (1971). Synthesis, intracellular transport, and discharge of secretory proteins in stimulated pancreatic exocrine cells.J. Cell Biol. 50, 135–58.

    Google Scholar 

  • KEENAN, T. W. & MORRÉ, D. J. (1970). Phospholipid class and fatty acid composition of Golgi apparatus isolated from rat liver and comparison of other cell fractions.Biochem. 9, 19–25.

    Google Scholar 

  • LOWRY, O. H., ROSEBROUGH, N. J., FARR, A. L., & RANDALL, R. J. (1951). Protein measurement with the Folin Phenol Reagent.J. biol. Chem. 193, 265–8.

    Google Scholar 

  • MELDOLESI, J. (1974). Dynamics of cytoplasmic membranes in guinea pig pancreatic acinar cells. I. Synthesis and turnover of membrane proteins.J. Cell Biol. 61, 1–13.

    Google Scholar 

  • MELDOLESI, J., JAMIESON, J. D. & PALADE, G. E. (1971). Composition of cellular membranes in the pancreas of the guinea pig. II. Lipids.J. Cell Biol. 49, 130–49.

    Google Scholar 

  • MITCHELL, R. H. & HAWTHORNE, J. N. (1965). The site of diphosphoinositide synthesis in rat liver.Biochem. biophys. Res. Commun. 21, 332–8.

    Google Scholar 

  • MORRÉ, D. J., KEENAN, T. W. & HUANG, C. M. (1974). Membrane flow and differentiation: origin of Golgi apparatus membranes from endoplasmic reticulum. In:Cytopharmacology of Secretion. (eds. B. Ceccarelli, J. Meldolesi and F. Clementi.) Advances in Cytopharmacology. Vol. 2. pp. 107–125. New York: Raven Press.

    Google Scholar 

  • NOVIKOFF, A. B. & NOVIKOFF, P. M. (1977). Cytochemical contributions to differentiating GERL from the Golgi apparatus.Histochem. J. 9, 525–51.

    Google Scholar 

  • PALADE, G. E. (1959). Functional changes in the structure of cell components. In:Subcellular Particles (ed. T. Hayashi.) pp. 64–83, New York: Ronald Press.

    Google Scholar 

  • PENNINGTON, R. J. (1961). Biochemistry of dystrophic muscle.Biochem. J. 80, 649–54.

    Google Scholar 

  • SCHNEIDER, W. C. (1957). Determination of nucleic acids in tissue by pentose analysis. In:Methods in Enzymology, Vol. 3 (eds. S. P. Colowick and N. P. Kaplan), pp. 680–684 New York, London: Academic Press.

    Google Scholar 

  • SHORE, G. E. & TATA, J. R. (1977). Two fractions of rough endoplasmic reticulum from rat liver. I. Recovery of rapidly sedimenting endoplasmic reticulum in association with mitochondria.J. Cell Biol. 72, 714–25.

    Google Scholar 

  • SKIPSKI, V. P., PETERSON, R. F. & BARCLAY, M. (1964). Quantitative analysis of phospholipids by thin-layer chromatography.Biochem. J. 90, 374–8.

    Google Scholar 

  • SMITH, C. S. & GUTMANN, L. (1953). Measurement of internal boundaries in three dimensional structures by random sectioning.Trans. AIME 197, 81–2.

    Google Scholar 

  • STAHL, W. L. & TRAMS, E. G. (1968). Synthesis of lipids of liver plasma membranes. Incorporation of acyl-coenzyme A derivatives into membrane lipidsin vitro.Biochem. biophys. Acta 163, 459–71.

    Google Scholar 

  • WALLACH, D., KIRSCHNER, N. & SCHRAMM, M. (1975). Non-parallel transport of membrane proteins and content proteins during assembly of the secretory granule in rat parotid gland.Biochem. biophys. Acta 375, 87–105.

    Google Scholar 

  • WATTIAUX, R. & De DUVE, C. (1956). Tissue fractionation studies 7. Release of bound hydrolases by means of Triton X-100.Biochem. J. 63, 606–8.

    Google Scholar 

  • WILLIAMS, M. A. (1977). Quantitative methods in biology. In:Practical Methods in Electron Microscopy. (ed. A. M. Glavert) Vol. 6. Amsterdam: North Holland.

    Google Scholar 

  • WILLIAMS, M. A. & COPE, G. H. (1973). A system for the study of zymogen granule genesisin vitro.J. Anat. (London) 116, 431–44.

    Google Scholar 

  • WILLIAMS, M. A., PRATTEN, M. K. & COPE, G. H. (1975). The application of stereological methods to the analysis of the purity of subcellular fractions.J. Anat. (Lond.) 119, 419.

    Google Scholar 

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Authors and Affiliations

  1. Department of Human Biology and Anatomy, University of Sheffield, UK

    M. A. Williams, M. K. Pratten, J. W. Turner & G. H. Cope

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  1. M. A. Williams
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  2. M. K. Pratten
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  3. J. W. Turner
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  4. G. H. Cope
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Williams, M.A., Pratten, M.K., Turner, J.W. et al. Comparative studies on the nature of purified cytomembranes of the rabbit parotid gland. Histochem J 11, 19–50 (1979). https://doi.org/10.1007/BF01041264

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  • DOI: https://doi.org/10.1007/BF01041264

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