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The amino acid sequence of hemoglobin III from the symbiont-harboring clamLucina pectinata

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Abstract

The cytoplasmic hemoglobin III from the gill of the symbiont-harboring clamLucina pectinata consists of 152 amino acid residues, has a calculated Mm of 18,068, including heme, and has N-acetyl-serine as the N-terminal residue. Based on the alignment of its sequence with other vertebrate and nonvertebrate globins, it retains the invariant residues Phe45 at position CD1 and His98 at the proximal position F8, as well as the highly conserved Trp16 and Pro39 at positions A12 and C2, respectively. The most likely candidate for the distal residue at position E7 is Gln66.Lucina hemoglobin III shares 95 identical residues with hemoglobin II (J. D. Hockenhull-Johnsonet al., J. Prot. Chem. 10, 609–622, 1991), including Tyr at position B10, which has been shown to be capable of entering the distal heme cavity and placing its hydroxyl group within a 2.8 Å of the water molecule occupying the distal ligand position, by modeling the hemoglobin II sequence using the crystal structure of sperm whale metmyoglobin. The amino acid sequences of the twoLucina globins are compared in detail with the known sequences of mollusc globins, including seven cytoplasmic and 11 intracellular globins. Relative to 75% homology between the twoLucina globins (counting identical and conserved residues), both sequences have percent homology scores ranging from 36–49% when compared to the two groups of mollusc globins. The highest homology appears to exist between theLucina globins and the cytoplasmic hemoglobin ofBusycon canaliculatum.

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Authors and Affiliations

  1. Department of Physiology, Wayne State University School of Medicine, 48201, Detroit, Michigan

    Jerrolynn D. Hockenhull-Johnson, Mary S. Stern, Serge N. Vinogradov & Daniel A. Walz

  2. Department of Physiology and Biophysics, Albert Einstein College of Medicine, 10461, Bronx, New York

    Jonathan B. Wittenberg

  3. Enrico Fermi Institute, University of Chicago, 60637, Chicago, Illinois

    Oscar H. Kapp

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  1. Jerrolynn D. Hockenhull-Johnson
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  2. Mary S. Stern
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  4. Serge N. Vinogradov
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  6. Daniel A. Walz
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Hockenhull-Johnson, J.D., Stern, M.S., Wittenberg, J.B. et al. The amino acid sequence of hemoglobin III from the symbiont-harboring clamLucina pectinata. J Protein Chem 12, 261–277 (1993). https://doi.org/10.1007/BF01028189

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