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Transformation ofBacillus subtilis with the treatment by alkali cations

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Summary

ExposingBacillus subtilis cultures to high concentrations of alkali cations, especially K+, allows efficient transformation by plasmids. The method allows transformation with unfractionated plasmid DNA, monomeric plasmid DNA as well as linear plasmid DNA.B. subtilis strains, not amenable to natural transformation, were also transformed by the present method.

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References

  • Anagnostopoulos, C., and Spizizen, J. (1961) J. Bacteriol. 81, 741–746.

    Google Scholar 

  • Birnboim, H.C. and Doly, J. (1979) Nucl. Acids Res. 7, 1513–1523.

    Google Scholar 

  • Canosi, U., Morelli, G. and Trautner, T.A. (1978) Mol. Gen. Genet. 166, 259–267.

    Google Scholar 

  • Chang, S. and Cohen, S.N. (1979) Mol. Gen. Genet. 168, 111–115.

    Google Scholar 

  • Contente, S. and Dubnau, D. (1979) Mol. Gen. Genet. 167, 251–258.

    Google Scholar 

  • Ehrlich, S.D. (1977) Proc. Natl. Acad. Sci. USA 74, 1680–1682.

    Google Scholar 

  • Gryczan, T.J., Contente, S. and Dubnau, D. (1978) J. Bacteriol. 134, 318–329.

    Google Scholar 

  • Ito, H., Fukuda, Y., Murata, K. and Kimura, A. (1983) J. Bacteriol. 153, 163–168.

    Google Scholar 

  • Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor N.Y.

    Google Scholar 

  • Mottes, M., Grandi, G., Sgaramella, V., Ganosi, U., Morelli, G., and Trautner, T.A. (1979) Mol. Gen. Genet. 174, 281–286.

    Google Scholar 

  • Notani, N.K. and Setlow, J. (1977) In: W.E. Cohen (ed) Progress in nucleic acid research and molecular biology vol. 14, Academic Press Inc, New York, pp. 39–100.

    Google Scholar 

  • Vogelstein, B. and Gillepsie, D. (1979) Proc. Natl. Acad. Sci. USA 76, 615–619.

    Google Scholar 

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Authors and Affiliations

  1. Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 227, Yokohama, Japan

    Takashi Ano, Akira Kobayashi & Makoto Shoda

Authors
  1. Takashi Ano
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  2. Akira Kobayashi
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  3. Makoto Shoda
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Ano, T., Kobayashi, A. & Shoda, M. Transformation ofBacillus subtilis with the treatment by alkali cations. Biotechnol Lett 12, 99–104 (1990). https://doi.org/10.1007/BF01022423

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  • DOI: https://doi.org/10.1007/BF01022423

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