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Production of rosmarinic acid from perfusion culture ofAnchusa officinalis in a membrane-aerated bioreactor

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Summary

In this study, a perfusion fermentation ofAnchusa officinalis was carried out in a stirred tank bioreactor integrated with an internal cross-flow filter. Bubble-free aeration via microporous membrane fibers was used to provide oxygen. A two-stage culture was successfully conducted in this reactor without filter fouling. In a 17 day fermentation, a cell density of 26 g dw/I and a rosmarinic acid productivity of 94 mg/l day were achieved. This productivity is three times that obtained in a batch culture.

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References

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  • Su, W. W., Caram, H. S., and Humphrey, A. E. (1991). Optimal design of the tubular microporous membrane aerator for shear sensitive cell cultures. Accepted for publication inBiotechnology Progress.

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Authors and Affiliations

  1. Department of Agricultural Engineering, University of Hawaii, 96822, Honolulu, HI, USA

    Wei Wen Su

  2. Department of Chemical Engineering, Lehigh University, 18015, Bethlehem, PA, USA

    Arthur E. Humphrey

Authors
  1. Wei Wen Su
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  2. Arthur E. Humphrey
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Su, W.W., Humphrey, A.E. Production of rosmarinic acid from perfusion culture ofAnchusa officinalis in a membrane-aerated bioreactor. Biotechnol Lett 13, 889–892 (1991). https://doi.org/10.1007/BF01022093

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  • DOI: https://doi.org/10.1007/BF01022093

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