Summary
Phenylethyl-β-D-galactoside (PEGal) synthesis by transglycosylation can be catalysed by Aspergillus oryzae β-galactosidase and was studied using high concentrations of glycosyl donor (lactose). The amount of PEGal formed reached a maximum value after 1Oh in the presence of 0.5M lactose. Beyond this time, PEGal was slowly hydrolysed. LiCl between 0.4M and 0.7M in the reaction medium prevented the progressive disappearance of the product.
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Fortun, Y., Colas, B. Lithium chloride effect on phenylethyl-B-D-galactoside synthesis by Aspergillus oryzae B-D-galactosidase in the presence of high lactose concentration. Biotechnol Lett 13, 863–866 (1991). https://doi.org/10.1007/BF01022087
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DOI: https://doi.org/10.1007/BF01022087