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Expression of human interleukin-2 from native and synthetic genes inE. coli: No correlation between major codon bias and high level expression

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Summary

Expression of human IL-2 from the cDNA and synthetic gene was compared using theE. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.

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Authors and Affiliations

  1. Department of Biochemistry, College of Medicine, University of Ulsan, 388-1 Pungnap-dong, Songpa-gu, 138-040, Seoul, Korea

    Hee-Won Lee & Doe Sun Na

  2. Asan Institute for Life Sciences, College of Medicine, University of Ulsan, 388-1 Pungnap-dong, Songpa-gu, 138-040, Seoul, Korea

    In-Sung Song

  3. Genetic Engineering Institute, Korea Institute of Science and Technology, San-1 Uheon-dong, Yuseong-gu, 305-333, Daejun, Korea

    Jae-Hoon Joo, Seongman Kang, Jong-Bum Kwon & Moon H. Han

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  1. Hee-Won Lee
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  2. Jae-Hoon Joo
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  3. Seongman Kang
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  4. In-Sung Song
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  5. Jong-Bum Kwon
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Lee, HW., Joo, JH., Kang, S. et al. Expression of human interleukin-2 from native and synthetic genes inE. coli: No correlation between major codon bias and high level expression. Biotechnol Lett 14, 653–658 (1992). https://doi.org/10.1007/BF01021637

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