Abstract
The NAD(P)H-dependent culture fluorescence of immobilizedClostridium acetobutylicum was followed during a three-part fermentation involving product formation on minimal and nitrogen-free media. The fluorescence signal, together with a knowledge of the metabolic pathways ofCl. acetobutylicum, provices information on the state and intracellular activities of the immobilized bacteria not readily found by other methods.
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Reardon, K.F., Scheper, T. & Bailey, J.E. In situ fluorescence monitoring of immobilizedClostridium acetobutylicum . Biotechnol Lett 8, 817–822 (1986). https://doi.org/10.1007/BF01020831
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DOI: https://doi.org/10.1007/BF01020831