Summary
The growth of mononuclear human peripheral blood cells from five patients with aplastic anemia (AA) was tested in diffusion chambers over a period of 13 days. Considerable differences were seen in the total cell number compared with controls. The total cell number of three patients with AA was markedly lower than in the control group (eight controls). The differentiation of the cells showed considerable differences in the growth patterns of the lymphocytes and in granulopoiesis; in three of five patients with AA no granulopoiesis was observed.
Zusammenfassung
Das Wachstum mononukleärer Zellen des peripheren Blutes von fünf Patienten mit aplastischer Anämie (AA) wurde über einen Zeitraum von 13 Tagen mittels der Diffusionskammerkultur untersucht. Deutliche Unterschiede zeigten sich beim Vergleich der Gesamtzellzahl während der Kulturperiode. Die Gesamtzellzahlen von drei Patienten mit aplastischer Anämie lagen wesentlich unter der Vergleichsgruppe (acht Normalpersonen). Bei der Differenzierung ergaben sich deutliche Abweichungen im Wachstumsmuster der Lymphozyten, besonders aber der Granulopoese, die bei drei Patienten mit AA im Beobachtungszeitraum nicht nachweisbar war.
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Literatur
Ascensao J, Kagan W, Malcolm M, Rajendra P, Hansen J, Robert G (1976) Aplastic Anemia: Evidence for an immunological mechanism. Lancet 27: 669–671
Benestad MD (1970) Formation of granulocytes and macrophages in diffusion chamber cultures of mouse blood leucocytes. Scand J Haematol 7: 279–288
Boggs DR, Boggs SS (1976) The Pathogenesis of Aplastic Anemia: A defective pluripotent 450 L. Boninget al. hematopoietic Stem Cell with inappropriate balance of differentiation and self-replication. Blood 48: 71–76
Boyum A (1968) Isolation of mononuclear cells and granulocytes from human peripheral blood. Scand J Clin Lab Invest 21: 77–89
Boyum A, Boecker W, Carsten AL, Cronkite EP (1972) Proliferation of human bone marrow cells in diffusion chambers implanted into normal or irradiated mice. Blood 40: 163–173
Cronkite EP, Boecker W, Carsten AL, Chikkappa G, Joel D, Laissue J, Öhl S (1974) The use of diffusion chamber cultures in the study of normal and leucémie cell proliferation in man. In: Robinson WA (ed) Hemopoiesis in culture. National Cancer Institute, Bethesda, MD, Washington, 185–193
Cronkite EP, Carsten AL, Chikkappa G, Laissue JA, Öhl S (1975) Culture of normal and leucémie cells in diffusion chambers. In: Fliedner TM, Perry S (eds) Advances in the Biosciences 14. Pergamon Press/F Vieweg u. Sohn, Braunschweig, 273–286
Dicke KA, Löwenberg B (1975) In vitro analysis of pancytopenia: Its relevance to the clinic. In: Fliedner TM, Perry S (eds) Advances in the Biosciences 14. Pergamon Press/F Vieweg u. Sohn, Braunschweig, 259–272
Ershler WB, Ross J, Finlay JL, Shahidi NT (1980) Bone-marrow microenvironment defect in congenital hypoplastic anemia. N Engl J Med 302: 1321–1327
Fitchen JH, Martin JC (1978) Recent developments in understanding the pathogenesis of aplastic anemia. Am J Hematol 5: 365–372
Good RA (1977) Aplastic anemia-suppressor lymphocytes and hematopoiesis. N Engl J Med 296: 41–42
Hoelzer D, Kurrle E, Ertl U, Milewski A (1974) Growth of human leukaemic peripheral blood cells in diffusion chambers. Eur J Cancer 10: 579–589
Kern P, Heit W, Kubanek B, Heimpel H (1977) Granulocytic colony-forming cells in vitro: II. Patients with aplastic anemia. Blut 34: 406–408
Kurnick JE, Robinson WA, Dickey CA (1971) In vitro granulocytic colony-forming potential of bone marrow from patients with granulocytopenia and aplastic anemia. Proc Soc Exp Biol Med 137: 917–920
Kurrle E, Hoelzer D, Schmücker H (1979) Culture of human haemopoietic cells in diffusion chambers. I. Growth of normal peripheral blood cells. Blut 38: 383–389
Ragab AH, Gilkerson E, Christ WM, Phelan E (1976) Granulopoiesis in childhood aplastic anemia. J Pediatr 88: 790–794
Singer JW, Brown JE (1978) In vitro marrow cultures techniques in aplastic anemia and related disorders. Clin Haematol 7: 487–499
Squires DJP (1975) The Growth of Human Bone Marrow in Diffusion Chambers. Br J Haematol 29: 89–97
Tigges FJ, v Heyden HW, Waller HD (1977) Kulturverhalten weißer Blutzellen bei Patienten mit Haarzell-Leukämie in der Diffusionskammer. Blut 35: 103–113
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Böning, L., Lau, B. & Wilmanns, W. Kultur mononukleärer Blutzellen von Patienten mit aplastischer Anämie in der Diffusionskammer. Blut 41, 445–450 (1980). https://doi.org/10.1007/BF01007769
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DOI: https://doi.org/10.1007/BF01007769