Summary
The complex carbohydrates in the epithelium of the goat prostate were studied with a battery of light microscopical histochemical methods that included peroxidase-labelled lectin-diaminobenzidine (PO-LT-DAB) procedures. The secretory epithelial cells were found to be of three types: (a) mucus-producing cells, (b) protein-secreting cells, and (c) cells intermediate between (a) and (b). All these cell types contained variable amounts of neutral and acidic complex carbohydrates. The histophysiological significances of these substances in the glandular cells are discussed with special reference to the function of the accessory sex glands.
Similar content being viewed by others
References
Aumüller, G. (1973) Über Sialomucin-produzierende Zellen der Prostata.Verh. Anat. Ges. 67, 233–43.
Aumüller, G. (1979) Prostate gland. InHandbuch der Mikroskopischen Anatomie des Menschen, VII/6, Prostate gland and seminal vesicles (edited byAumüller, G.), pp. 53–182. Berlin, Heidelberg, New York: Springer-Verlag.
Brandes, D. (1974) Fine structure and cytochemistry of male sex accessory organs. InMale Accessory Sex Organs (edited byBrandes, D.), pp. 17–114. New York, London: Academic Press.
Cavazos, L. F. (1975) Fine structure and functional correlates of male accessory sex glands of rodents. InHandbook of Physiology (edited byHamilton, D. W. andGreep, R. O.), Vol. 5, pp. 358–81. Washington: American Physiological Society.
Collard, J. G. &Temmink, J. H. M. (1974) Binding and cytochemical detection of cell-bound concanavalin A.Exp. Cell Res. 86, 81–6.
Dellman, H.-D. &Wrobel, K.-H. (1976) Male reproductive system. InTextbook of Veterinary Histology (edited byDellman, H.-D. andBrown, E. M.) pp. 291–318. Philadelphia: Lea & Febiger.
Franks, L. M., O'shea, J. D. &Thomson, A. E. R. C. (1964) Mucin in the prostate. A histochemical study in normal glands, latent, clinical and colloid cancers.Cancer 17, 983–91.
Geuze, J. J. &Slot, J. W. (1978) Synthesis and secretion of glycoprotein in rat bulbo-urethral (Cowper's) glands.Am. J. Anat. 152, 391–418.
Halbhuber, J. &Geyer, G. (1965) Histochemischer Nachweis von Sialomucin in den Glandulae bulbourethrales.Acta Histochem. 21, 148–49.
Kiernan, J. A. (1975) Localization of α-d-glucosyl and α-d-mannosyl groups, of mucosubstances with concanavalin A and horseradish peroxidase.Histochemistry 44, 39–45.
Leppi, T. J. (1968) Morphochemical analysis of mucous cells in the skin and slime glands of hagfishes.Histochemie 15, 68–78.
Lev, R. &Spicer, S. S. (1964) Specific staining of sulfate groups with alcian blue at low pH.J. Histochem. Cytochem. 12, 309.
Mann, T. (1964)The Biochemistry of Semen and of the Male Reproductive Tract. London: Methuen.
Mann, T. (1975) Biochemistry of semen. InHandbook of Physiology (edited byHamilton, D. W. andGreep, R. O.), Vol. 5, pp. 461–71. Washington: American Physiological Society.
Mcmanus, J. F. A. &Mowry, R. W. (1958) Effects of fixation on carbohydrate histochemistry.J. Histochem. Cytochem. 6, 309–16.
Mowry, R. W. (1963) The special value of methods that colour with acidic and hydroxyl groups in the histochemical study of mucins. With revised directions for the colloidal iron stain, the use of Alcian Blue 8 GX and their combination with the periodic acid-Schiff reaction.Ann. N.Y. Acad. Sci. 106, 402–23.
Nickel, R., Schummer, A. &Seiferle, E. (1967)Lehrbuch, der Anatomie der Haustiere. Bd. II, pp. 307–54. Berlin & Hamburg: Paul Parey.
Nielsen, E. H. (1976) The bulbourethral gland of the rat. Fine structure and histochemistry.Anat. Anz. 139, 254–63.
Pearse, A. G. E. (1968)Histochemistry, Theoretical, and Applied. Vol. 1. London: J. & A. Churchill.
Spicer, S. S. (1960) A correlative study of the histochemical properties of rodent acid mucopolysaccharides.J. Histochem. Cytochem. 8, 18–35.
Spicer, S. S. (1965) Diamine methods for differentiating mucosubstances histochemically.J. Histochem. Cytochem. 13, 211–43.
Spicer, S. S., Horn, R. G. &Leppi, T. J. (1967) Histochemistry of connective tissue mucopolysaccharides. InThe Connective Tissue (edited byWagner, B. M. andSmith, D. E.) pp. 251–303. Baltimore: Williams & Wilkins.
Stoward, P. J., Spicer, S. S. &Miller, R. L. (1980) Histochemical reactivity for peanut lectin-horseradish peroxidase conjugate.J. Histochem. Cytochem. 28, 939–90.
Tsukise, A. &Yamada, K. (1981a) The histochemistry of complex carbohydrates in the epithelium lining the ventral prostate of the rat.Histochemistry 72, 215–27.
Tsukise, A. &Yamada, K. (1981b) The histochemistry of complex carbohydrates in the scrotum of the boar.Histochemistry 72, 511–21.
Tsukise, A. & Yamada, K. (1983) The histochemistry of complex carbohydrates in the epithelium lining the prostate, seminal vesicle and bulbourethral glands of the boar.Proceedings of the 87th Meeting Japanese Association of Anatomists 73.
Tsukise, A., Sugawa, Y. &Yamada, K. (1979) Histochemistry of carbohydrates in the epithelium lining the bulbourethral gland of the rat.Acta anat 105, 529–38.
Wrobel, K.-H. (1970) Feinstruktur und histochemie der Glandula bulbourethralis der Ziege.Z. Zellforsch. 108, 582–96.
Wrobel, K.-H. (1972) Histochemische Untersuchungen am Prostata-parenchym der Ziege.Zbl. Vet. Med. C1, 64–72.
Wrobel, M. (1972) Histochemische und elektronenmikroskopische Untersuchungen an der Prostata von Hund und Katze.Zbl. Vet. Med. C1, 93–116.
Yamada, K. (1972) Histochemical and cytochemical methods for the study of mucosaccharides.Protein, Nucleic Acid and Enzyme 17, 775–90.
Yamada, K. (1973) The effect of digestion withStreptomyces hyaluronidase upon certain histochemical reactions of hyaluronic acid-containing tissue.J. Histochem. Cytochem. 21, 794–803.
Yamada, K. &Hoshino, M. (1972) Morphochemical, analysis of epithelial mucosubstances in the gall bladder of the fowl (Gallus domesticus).Histochemie 29, 120–28.
Yamada, K. &Shimizu, S. (1977) The histochemistry of galactose residues of complex carbohydrates as studied by peroxidase-labelledRicinus communis agglutinin.Histochemistry 53, 143–56.
Yamada, K. &Shimizu, S. (1979) The use of peroxidase-labelledLimulus polyphemus agglutinin for the histochemistry of sialic acid-containing glycoproteins in light microscopy.Histochem. J. 11, 457–71.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tsukise, A., Yamada, K. Complex carbohydrates in the secretory epithelium of the goat prostate. Histochem J 16, 311–319 (1984). https://doi.org/10.1007/BF01003614
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01003614