Abstract
The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.
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Special issue dedicated to Dr. Sidney Ochs.
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Hodes, M.E., Dlouhy, S.R., Wei, J.J. et al. cDNA approaches to isolation of the mouse mutant weaver gene. Neurochem Res 19, 1359–1362 (1994). https://doi.org/10.1007/BF00972463
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DOI: https://doi.org/10.1007/BF00972463