Abstract
Recently, the partial structural characterization of a novel bovine brain protein was reported (1). Because of its mitogenic activity for vascular endothelial cells and its ability to strongly bind heparin it was termed heparin-binding brain mitogen (HBBM). Although HBBM shares these properties with members of the fibroblast growth factor (FGF) family of growth factors, its aminoterminal sequence is not homologous to that of the FGFs. Now, we report the isolation and partial structural characterization of HBBMs from human, rat and chick brain. Proteins were isolated by tissue extraction at pH 4.5, ammonium sulfate precipitation, cation exchange chromatography, heparin-Sepharose affinity chromatography and reverse-phase HPLC. The amino-terminal sequences of the HBBMs from human, bovine and rat brain are identical, whereas that of chick HBBM reveals a single amino acid substitution. The high sequence homology among the HBBMs from different species suggests an important biological role of the protein.
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Böhlen, P., Gautschi-Sova, P., Albrecht, U., Lehmann, S., and Huber, D. 1988. Isolation and partial structural characterization of a novel heparin-binding, non-FGF-like endothelial cell growth factor. J. Cell. Biochem. Suppl. 12A:221.
Böhlen, P., Baird, A., Esch, F., Ling, N., and Gospodarowicz, D. 1984. Isolation and partial molecular characterization of pituitary fibroblast growth factor. Proc. Natl. Acad. Sci. USA 81:5364–5368.
Esch, F., Baird, A., Ling, N., Ueno, N., Hill, F., Denoroy, L., Klepper, R., Gospodarowicz, D., Böhlen, P., and Guillemin, R. 1985. Primary structure of bovine pituitary fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine acidic FGF. Proc. Natl. Acad. Sci. USA 82:6507–6511.
Böhlen, P., Esch, F., Baird, A., and Gospodarowicz, D. 1985. Acidic fibroblast growth factor (FGF) from bovine brain: aminoterminal sequence and comparison with basic FGF. EMBO J. 4:1951–1956.
Gimenez-Gallego, G., Rodkey, J., Bennett, C., Rios-Candelore, M., DiSalvo, J., and Thomas, K. 1985. Brain-derived acidic fibroblast growth factor: complete amino acid sequence and homologies. Science 230:1385–1388.
Dickson, C., and Peters, G. 1987. Potential oncogene product related to growth factors. Nature 326:833.
Delli Bovi, P., Curatola, A., Kern, F., Greco, A., Ittmann, M., and Basilico, C. 1987. An oncogene isolated by transfection of Kaposi's sarcoma DNA encodes a growth factor that is a member of the FGF family. Cell 50:729–737.
Yoshida, T., Miyagawa, K., Odagiri, H., Sakamoto, H., Litie, P., Terada, M., and Sugimura, T. 1987. Genomic sequence of hst, a transforming gene encoding a protein homologous to fibroblast growth factors and the int-2-encoded protein. Proc. Natl. Acad. Sci. USA 84:7305–09.
Zhan, X., Bates, B., and Goldfarb, M. 1988. The human FGF-5 oncogene encodes a novel protein related to fibroblast growth factors. Mol. Cell. Biol. 8:3487–3495.
Ishikawa, F., Miyazono, K., Hellman, U., Drexler, H., Wernstedt, C., Hagiwara, K., Usuki, K., Takaku, F., Risau, W., and Heldin, C. H. 1989. Identification of angiogenic activity and the cloning and expression of platelet-derived endothelial growth factor. Nature 338:557–561.
Ferrara, N., and Henzel, W. J. 1989. Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells. Biochem. Biophys. Res. Commun. 161:851–858.
Gospodarowicz, D., Cheng, J., Lui, G. M., Baird, A., and Böhlen, P. 1984. Isolation of brain fibroblast growth factor by heparin-Sepharose affinity chromatography: identity with pituitary fibroblast growth factor. Proc. Natl. Acad. Sci. USA 81:6963–6967.
Gautschi, P., Fràter-Schroeder, M., and Böhlen, P. 1986. Partial molecular characterization of endothelial cell mitogens from human brain: acidic and basic fibroblast growth factors. FEBS Lett. 204:203–207.
Gautschi-Sova, P., Müller, T., and Böhlen, P. 1986. Amino acid sequence of human acidic fibroblast growth factor. Biochem. Biophys. Res. Commun. 140:874–880.
Risau, W., Gautschi-Sova, P., and Böhlen, P. 1988. Endothelial cell growth factors in embryonic and adult chick brain are related to human acidic fibroblast growth factor. EMBO J 7:959–962.
Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685.
Gospodarowicz, D., Massoglia, S., Cheng, J., Lui, G. M., and Böhlen, P. 1985. Isolation of pituitary fibroblast growth factor by fast protein liquid chromatography (FPLC): Partial chemical and biological characterization. J. Cell. Physiol. 122:323–332.
Gautschi, P., Fràter-Schroeder, M., Müller, T., and Böhlen, P. 1986. Chemical and biological characterization of a truncated form of acidic fibroblast growth factor from bovine brain. Eur. J. Biochem. 160:357–361.
Böhlen, P. 1989. Fibroblast growth factor. Pages 204–228, in Sorg, C. (ed.), Macrophage-derived Cell Regulatory Factors,Cytokines. Vol. 1 Karger, Basel
Rauvala, H. 1989. An 18-kd heparin-binding protein of developing brain that is distinct from fibroblast growth factors. EMBO J. 8:2933–2941.
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Special issue dedicated to Dr. Sidney Udenfriend.
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Huber, D., Gautschi-Sova, P. & Böhlen, P. Amino-terminal sequences of a novel heparin-binding protein from human, bovine, rat, and chick brain: High interspecies homology. Neurochem Res 15, 435–439 (1990). https://doi.org/10.1007/BF00969930
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DOI: https://doi.org/10.1007/BF00969930