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Effect of GABAmimetics on electrocorticographic spike discharges induced by guanidinoethanesulfonic acid (amidino-taurine) in the rat

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Abstract

The effect of guanidinoethanesulfonic acid (GES) on rat electrocorticograms (ECoG) and the effects of γ-aminobutyric acid (GABA) and GABA-agonists on the ECoG changes induced by GES were studied. Sporadic spike discharges began 2–5 min after 1 μmol GES/10 μl on filter paper was applied to the pia mater of the left sensorimotor cortex; spike discharges extended to the opposite cerebral hemisphere 60 min after the onset of the ipsilateral spike discharges. The spike discharges with a frequency of 5–10 spikes/min lasted until the end of the 4 hour recording. The induced spike discharges were suppressed when the original GES soaked filter paper was replaced by one containing GES (1 μmol) supplement combined with taurine (1 μmol/10 μl). GABA (1 μmol) and its receptor agonist, muscimol (10nmol) and (3R)-(−)-4-amino-3-hydroxybutyric acid (1 μmol) also suppressed the GES-induced spike discharges when applied topically. Diazepam (DZP) (10 mg/kg) suppressed the GES-induced spike discharges 10 min after i.p. injection, but phenobarbital (20 mg/kg) increased the frequency and voltage of spike discharges 100 min following subcutaneous administration. Intraperitoneal injection of either valproate (200 mg/kg) or phenytoin (25 mg/kg), after the completion of the spike discharges, showed no effect. These findings suggest that neurotransmission or neuromodulatory effects of taurine participate in GES-induced seizure activity, and that GABAA and DZP receptors may play a role in the mechanism that suppresses GES-induced seizures.

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Authors and Affiliations

  1. Department of Neuroscience, Institute of Molecular and Cellular Medicine, Okayama University Medical School, 2-5-1 Shikata-cho, 700, Okayama, Japan

    Isao Yokoi, Hideaki Kabuto & Akitane Mori

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  1. Isao Yokoi
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  2. Hideaki Kabuto
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  3. Akitane Mori
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Yokoi, I., Kabuto, H. & Mori, A. Effect of GABAmimetics on electrocorticographic spike discharges induced by guanidinoethanesulfonic acid (amidino-taurine) in the rat. Neurochem Res 18, 533–538 (1993). https://doi.org/10.1007/BF00967258

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