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High-affinity uptake of γ-[3H]aminobutyric acid by isolated mouse oligodendrocytes in culture

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Abstract

Oligodendrocytes were isolated from mixed glial cultures of neonatal mouse forebrain and further grown in serum-free hormone supplemented culture medium. Cell populations were identified by indirect immunofluorescence using a range of specific antibodies, revealing a predominantly immature population of oligodendrocytes, the majority expressing the myelin glycolipids galactocerebroside and sulfatide on their plasma membrane. Astroglial contamination was found to be minimal. Simultaneous autoradiography and immunofluorescence demonstrated the presence of a transport system for the major inhibitory neurotransmitter GABA in the oligodendrocytes. The transport system was found to be energy, sodium and temperature dependent. Kinetic analysis revealed a high affinity system, with aK m of 6.27 μM and aV max of 0.714 nmol/min/mg protein, which is comparable to that found previously for CNS neurons and astrocytes.

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Author notes

  1. Richard Reynolds

    Present address: Department of Biochemistry, Imperial College, SW7 2AZ, London

Authors and Affiliations

  1. Department of Pediatrics, University of Berne, Inselspital, CH-3010, Berne, (Switzerland)

    Richard Reynolds, Christine Steffen & Norbert Herschkowitz

Authors
  1. Richard Reynolds
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  2. Christine Steffen
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  3. Norbert Herschkowitz
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Special Issue dedicated to Dr. E. M. Shooter and Dr. S. Varon.

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Reynolds, R., Steffen, C. & Herschkowitz, N. High-affinity uptake of γ-[3H]aminobutyric acid by isolated mouse oligodendrocytes in culture. Neurochem Res 12, 885–890 (1987). https://doi.org/10.1007/BF00966310

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