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The mechanism for the methionine mediated reduction of anthramycin yields inStreptomyces refuineus fermentations

  • Industrial Microbiology
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Summary

The addition of L-methionine to cultures ofStreptomyces refuineus producing anthramycin, results in an apparent decrease in the antibiotic titer. It has been shown that this effect is not due to the inhibition of tyrosinase, an enzyme implicated in anthramycin biosynthesis. The inhibitory effect also appears unlikely to be due to a regulatory role played by methionine in a non-precursor fashion. Experiments with radiolabeled anthramycin have revealed that the reduced yields of the antibiotic are due to the interaction of anthramycin with generated reactive metabolites produced in the methionine-supplemented cultures.

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Authors and Affiliations

  1. Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Kentucky, 40506, Lexington, Kentucky, USA

    C. Gairola & L. Hurley

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  1. C. Gairola
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  2. L. Hurley
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Gairola, C., Hurley, L. The mechanism for the methionine mediated reduction of anthramycin yields inStreptomyces refuineus fermentations. European J. Appl Microbiol. 2, 95–101 (1976). https://doi.org/10.1007/BF00942352

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  • DOI: https://doi.org/10.1007/BF00942352

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