Abstract
A 5S rRNA gene (5S DNA) from the coniferPinus radiata D. Don has been cloned and characterized at the nucleotide, genomic and chromosomal levels. Sequencing revealed a repeat unit of 524 base pairs which is present in approximately 3000 copies per diploid genome. Two-dimensional gel electrophoresis indicated that these copies are organized in tandem arrays of various length. Using in situ hybridization techniques, the tandem arrays appear to be present on all of the chromosomes. This complexity of chromosomal organization contrasts markedly with the few sites of uniform length found in angiosperm plants such as wheat, pea, and maize.
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Gorman, S.W., Teasdale, R.D. & Cullis, C.A. Structure and organization of the 5S rRNA genes (5S DNA) inPinus radiata (Pinaceae). Pl Syst Evol 183, 223–234 (1992). https://doi.org/10.1007/BF00940805
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DOI: https://doi.org/10.1007/BF00940805