Abstract
To improve the diagnosis ofPhytomonas infections in plants, we developed a polymerase chain reaction (PCR) assay using synthetic oligonucleotides complementary to conserved sequences of the 18S small subunit ribosomal (SSU) gene. From 10 ng upward of DNA of cultures ofPhytomonas isolated from plants, fruits, and insects, PCR amplified an 800-bp DNA band that, after restriction analysis and probe hybridization, proved to be of 18S rDNAPhytomonas origin. PCR was also done with sap samples of tomatoes experimentally infected withPhytomonas, yielding amplified 800-bp ribosomal DNA bands before any flagellate could be detected by microscopic examination of the fruit sap.
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Teixeira, M.M.G., Campaner, M. & Camargo, E.P. Detection of trypanosomatidPhytomonas parasitic in plants by polymerase chain reaction amplification of small subunit ribosomal DNA. Parasitol Res 80, 512–516 (1994). https://doi.org/10.1007/BF00932699
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DOI: https://doi.org/10.1007/BF00932699