Abstract
The pattern of infection inGlossina morsitans morsitans andG. m. centralis membrane-fed on eland, buffalo or goat blood mixed withTrypanosoma congolense orT. brucei was studied from day 1 to day 10. Tsetse were initially permissive vectors, with most flies harbouring infections of 104–105 parasites on day 3. However, after a second blood meal on day 3, flies cleared many infections withG. m. morsitans clearing more infections thanG. m. centralis. Infective feeds of goat blood consistently increased final infection rates by limiting the number of infections lost between days 3 and 6. In further experiments withG. m. morsitans only, this effect was replicated by feeding flies on erythrocytes but not on serum. These results suggest that compounds from some mammalian erythrocytes match the target specificity ofG. m. morsitans midgut lectins and, hence, have a protective effect on trypanosome establishment in the fly.
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References
Dipeolu OO (1975) Studies on the development ofTrypanosoma congolense in tsetse flies (Glossina: Diptera) and the factors affecting it. Acta Protozool 14:241–251
Gooding RH (1974) Digestive processes of haematophagous insects. V. Inhibitors of trypsin fromGlossina morsitans morsitans (Diptera: Glossinidae). Can Entomol 106:39–44
Ibrahim EAR, Ingram GA, Molyneux DH (1984) Haemagglutinins and parasite agglutinins in haemolymph and gut ofGlossina. Tropenmed Parasitol 35:151–156
Ingram GA, Molyneux DH (1988) Sugar specificities of anti-human ABO (H) blood group erythrocyte agglutinins (lectins) and haemolytic activity in the haemolymph and gut extracts of threeGlossina species. Insect Biochem 18:269–279
Kabayo JP, Langley PA (1985) The nutritional importance of dietary blood components for reproduction in the tsetse fly,Glossina morsitans. J Insect Physiol 31:619–624
Langley PA (1966) The effect of environment and host type on the rate of digestion in the tsetse flyGlossina morsitans Westw. Bull Entomol Res 57:39–48
Lehane MJ, Msangi AR (1991) Lectin and peritrophic membrane development in the gut ofGlossina m. morsitans and a discussion of their role in protecting the fly against trypanosome infection. Med Vet Entomol 5:495–501
Maudlin I (1991) Transmission of African trypanosomiasis: interactions among tsetse immune system, symbionts, and parasites. Adv Vector Res 7:117–148
Maudlin I, Kabayo JP, Flood MET, Evans DA (1984) Serum factors and the maturation ofTrypanosoma congolense infections inGlossina morsitans. Z Parasitenkd 70:11–19
Mihok S, Otieno LH, Darji N, Munyinyi D (1992) Influence ofD(+)-glucosamine on infection rates and parasite loads inTrypanosoma brucei infected tsetse flies (Glossina spp.). Acta Trop (Basel) 51:217–228
Mihok S, Olubayo RO, Darji N, Zweygarth E (1993a) The influence of host blood in infection rates inGlossina morsitans spp. infected withTrypanosoma congolense, T. brucei andT. simiae. Parasitology 107:41–48
Mihok S, Masaninga F, Munyoki E, Ndegwa P, Olubayo RO (1993b) Isolation ofTrypanosoma spp. from wild tsetse flies through procyclic expansion inGlossina morsitans centralis. Acta Trop (Basel)(in press)
Mihok S, Stiles JK, Mpanga E, Olubayo RO (1994) Relationships between protease activity, host blood and infection rates inGlossina morsitans sspp. infected withTrypanosoma congolense, T. brucei andT. simiae. Med Vet Entomol (in press)
Osir EO, Imbuga MO, Onyango P (1993) Inhibition ofGlossina morsitans midgut trypsin activity byD-glucosamine. Parasitol Res 79:93–97
Pospisil J, Kase F, Vahala J, Mouchova I (1984) Basic haematological values in antelopes. IV. A comparison and concluding remarks. Comp Biochem Physiol [A] 78:815–821
Rickman L, Kolala F (1982) Effects of some African game animal sera onTrypanosoma brucei rhodesiense andT. b. brucei clones. Tropenmed Parasitol 33:129–135
Stiles JK, Ingram GA, Wallbanks KR, Molyneux DH, Maudlin I, Welburn S (1990) Identification of midgut trypanolysin and trypanoagglutinin inGlossina palpalis sspp. (Diptera: Glossinidae). Parasitology 101:369–376
Van den Abbeele J, Decleir W (1992) Study of the vectorial capacity ofGlossina palpalis palpalis related to its digestive physiology and rearing conditions. IAEA-TECDOC-634. International Atomic Energy Agency, Vienna, pp 91–103
Welburn SC, Maudlin I, Ellis DS (1989) Rate of trypanosome killing by lectins in midguts of different species and strains ofGlossina. Med Vet Entomol 3:77–82
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Olubayo, R.O., Mihok, S., Munyoki, E. et al. Dynamics of host blood effects inGlossina morsitans sspp. infected withTrypanosoma congolense andT. brucei . Parasitol Res 80, 177–181 (1994). https://doi.org/10.1007/BF00932670
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DOI: https://doi.org/10.1007/BF00932670