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Interaction ofTrypanosoma cruzi with macrophages: effect of previous incubation of the parasites or the host cells with lectins

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Abstract

The effect of incubation with lectins of the macrophages or two evolutive stages ofTrypanosoma cruzi (noninfective epimastigotes and infective trypomastigotes) on the ingestion of the parasites by mouse peritoneal macrophages was studied. Lectins which bind to residues of mannose (Lens culinaris, LCA),n-acetyl-d-glucosamine orn-acetylneur-aminic acid (Triticum vulgaris, WGA), β-d-galactose (Ricinus communis, RCA),n-acetyl-d-galactosamine (Phaseolus vulgaris, PHA;Dolichos biflorus, DBA; andWistaria floribunda, WFA), fucose (Lotus tetragonolobus, LTA), andn-acetylneuraminic acid (Limulus polyphemus, LPA) were used. By lectin blockage we concluded that, α-d-mannose-like, β-d-galactose andn-acetyl-d-galactosamine (PHA, reagent) residues, located on the macrophage's surface are required for both epi- and trypomastigote uptake, whilen-acetylneuraminic acid and fucose residues, impede trypomastigote ingestion but do not interfere with epimastigote interiorization. Macrophages'n-acetyl-d-glucosamine residues are required for epimastigote uptake. On the other hand, from theT. cruzi surface, mannose residues prevent ingestion of epi- and trypomastigotes. Galactose residues participate in endocytosis of trypomastigotes, but hinder epimastigote interiorization. Exposedn-acetyl-d-glucosamine residues are required for uptake of the two evolutive forms.n-acetylneuraminic acid residues on the trypomastigote membrane prevent their endocytosis by macrophages. These results together with those reported previously showing the effect of monosaccharides on theT. cruzi-macrophage interaction, indicate that (a) sugar residues located on the parasite and on macrophage surface play some role in the process of recognition ofT. cruzi, (b) different macrophage carbohydrate-containing receptors are involved in the recognition of epimastigotes and trypomastigotes forms ofT. cruzi, (c)n-acetylneuraminic acid residues located on the surface of trypomastigotes or macrophages impede the interaction of the parasite with these host cells, and suggest that (d) sugar-binding proteins located on the macrophage surface participate in the recognition of β-d-galactose andn-acetyl-d-galactosamine residues located on the surface of trypomastigotes and exposed after blockage or splitting off ofn-acetylneuraminic acid residues. Some lectins which bind to macrophages and block the ingestion of parasites did not interfere with their adhesion.

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Authors and Affiliations

  1. Departamento de Ultraestrutura e Biologia Celular, Fundação Oswaldo Cruz, Avenida Brasil, 4365, Manguinhos, 21040, Rio de Janeiro, RJ., Brasil

    Tania C. de Araújo-Jorge

  2. Laboratório de Ultraestrutura Celular, Instituto de Biofisica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21941, Rio de Janeiro, RJ., Brasil

    Tania C. de Araújo-Jorge & Wanderley de Souza

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  1. Tania C. de Araújo-Jorge
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  2. Wanderley de Souza
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de Araújo-Jorge, T.C., de Souza, W. Interaction ofTrypanosoma cruzi with macrophages: effect of previous incubation of the parasites or the host cells with lectins. Z. Parasitenkd. 72, 153–171 (1986). https://doi.org/10.1007/BF00931143

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