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Effects of hypocaloric feeding and refeeding on myocardial Ca and ATP cycling in the rat

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Abstract

Hypocaloric feeding (HCF) depresses heart function causing cardiac atrophy, bradycardia, and decreased cardiac output. We tested the hypothesis that HCF results in decreased myocardial Ca- and ATP cycling. We reduced protein-calorie intake of adult rats by 20% for 7 days and then allowed them to recover for 3 days. Changes in ionized Ca concentration (nM/s) of 2.5% myocardial homogenates that were attributable to the Ca-ATPase pump and Ca-release channel (CRC), respectively, of the sarcoplasmic reticulum (SR) were depressed 41 and 85% by HCF from 61.6±9.4 and 24.7±3.3, to 36.1±2.8 and 3.6±2.9. Activity of the Ca-pump was restored after 3 days of refeeding, whereas the CRC remained 23% depressed (all p<0.05). Additionally, the CRC activity was inhibited to a 3-fold greater extent than controls by HCF, but was disinhibited within one day of refeeding. The greater effect on CRC than Ca-pump activity resulted in net Ca-uptake being unaffected by HCF. In addition to depression of Ca-cycling, ATP sythetase and total ATPase activities (IU/g), respectively, were depressed 20 and 15% by HCF from 174±19 and 51.3±3.8 to 140±15 and 43.7±4.7, but were restored to control values within one day of refeeding. We conclude that HCF produces a compensatory, reversible, and asymmetric downregulation and inhibition of Ca-cycling, with the CRC being preferentially affected.

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References

  1. Keys A, Henschel A, Taylor HL: The size and function of the human heart at rest in semi-starvation and in subsequent rehabilitation. Am J Physiol 150: 153–169, 1947

    Google Scholar 

  2. Bergman JW, Human DG, De Moor MMA, Schulz JM: Effect of kwashiorkor on the cardiovascular system. Arch Dis Childhood 63: 1359–1362, 1988

    Google Scholar 

  3. Alden PB, Madoff RD, Stahl TJ, Lakatua DJ, Ring WS, Cerra FB: Left ventricular function in malnutrition. Am J Physiol 253: H380-H387, 1987

    PubMed  Google Scholar 

  4. McGuirk SM, Bednarski RM, Clayton MK: Bradycardia in cattle deprived of food. J Am Vet Med Assoc 196: 894–896, 1990

    PubMed  Google Scholar 

  5. Nutter DO, Murray TG, Heymsfield SB, Fuller EO: The effect of chronic protein-calorie undernutrition in the rat on myocardial function and cardiac function. Circ Res 45: 144–152, 1979

    PubMed  Google Scholar 

  6. Cascarano J, Migler RA, Wilson MA: Starvation and refeeding in rats: effect on organismic respiration, cytoplasmic constituents of liver, and succinic dehydrogenase activity in liver, kidney, and heart. J Nutr 108: 1606–1615, 1978

    PubMed  Google Scholar 

  7. McR Russell D, Atwood HL, Jeejeebhoy KN: Nitrogen versus muscle calcium in the genesis of abnormal muscle function in malnutrition. J Parenteral Enteral Nutr 9: 415–421, 1985

    Google Scholar 

  8. Pichard C, Vaughan C, Struk R, Armstrong RL, Jeejeebhoy KN: The effect of dietary manipulations (fasting, hypocaloric feeding, and subsequent refeeding) on rat muscle energetics as assessed by nuclear magnetic resonance spectroscopy. J Clin Invest 82: 895–901, 1988

    PubMed  Google Scholar 

  9. de la Torre AM, Madapallimattam A, Cross A, Armstrong RL, Jeejeebhoy KN: Effect of fasting, hypocaloric feeding, and refeeding on the energetics of stimulated rat muscle as assessed by nuclear magnetic resonance spectroscopy. J Clin Invest 92: 114–121, 1993

    PubMed  Google Scholar 

  10. O'Brien PJ, Ling E, Williams H, Salerno T, Ianuzzo CD: Compensatory increase of myocardial sarcoplasmic reticulum Ca-ATPase activity in response to chronic metabolic overload. Can J Cardiol 4: 243–250, 1988

    PubMed  Google Scholar 

  11. Martin V, McCutcheon LJ, Poon L, Shen H, Cory CR, O'Brien PJ: Comparative mammal model of chronic rate overload: relationship of myocardial Ca-cycling to heart, metabolic and lipoperoxidation rates. Comp Biochem Physiol 106B: 453–461, 1993

    Google Scholar 

  12. O'Brien PJ, Ianuzzo CD, Moe GW, Howard RJ, Stopps TP, Armstrong PW: Rapid ventricular pacing in the dog: biochemical and physiological studies of heart failure. Can J Physiol Pharmacol 68: 34–39, 1990

    PubMed  Google Scholar 

  13. Cory CR, McCutcheon LJ, O'Grady M, Pang AW Geiger JW, O'Brien PJ: Compensatory downregulation of myocardial Ca channel in SR from dogs with heart failure. Am J Physiol 264: H926–37, 1993

    Google Scholar 

  14. Cory CR, Shen H, O'Brien PJ: Compensatory asymmetry in downregulation and inhibition of the Ca-cycle in congestive heart failure produced in dogs by idiopathic dilated cardiomyopathy and rapid ventricular pacing. J Mol Cell Cardiol 26: 173–184, 1994

    PubMed  Google Scholar 

  15. O'Brien PJ, Shen H, Weiler JE, Mirsalimi SM, Julian RJ: Myocardial calcium cycling defect in furazolidone cardiomyopathy. Can J Physiol Pharmacol 69: 1833–40, 1991

    PubMed  Google Scholar 

  16. Arai M, Matsui H, Periasamy M: Sarcoplasmic reticulum gene expression in cardiac hypertrophy and heart failure. Circ Res 74: 555–564, 1994

    PubMed  Google Scholar 

  17. Simonides WS and C van Hardeveld: An assay for sarcoplasmic reticulum Ca2+-ATPase activity in muscle homogenates. Anal Biochem 191: 321–331, 1990

    PubMed  Google Scholar 

  18. O'Brien PJ, O'Grady M, McCutcheon LJ, Nowack L, Horn RD, Mirsalimi SM, Julian RJ, Moe GW, Armstrong PW: Myocardial myoglobin deficiency in various animal models of congestive heart failure. J Mol Cell Cardiol 24: 721–730, 1992

    PubMed  Google Scholar 

  19. McCutcheon LJ, O'Grady M, Cory RJ, O'Brien PJ: Respiratory chain defect of myocardial mitochondria in idiopathic dilated cardiomyopathy of Doberman

  20. O'Brien PJ, Shen H, McCutcheon LJ, O'Grady M, Byrne PJ, Ferguson HW, Mirsalimi, Julian RJ, Sargeant JM, Tremblay RRM, Blackwell TE: Rapid, simple and sensitive microassay for skeletal and cardiac muscle myoglobin and hemoglobin: use in various animals indicates functional role of myohemoproteins. Mol Cell Biochem 112: 45–52, 1992

    PubMed  Google Scholar 

  21. O'Brien PJ, Moe GW, Nowack LM, Grima EA, Armstrong PW: Sarcoplasmic reticulum Ca-release channel and ATP synthesis activities are early myocardial markers of heart failure produced by rapid ventricular pacing in dogs. Can J Physiol Pharmacol. In Press.

  22. O'Brien PJ, Mirsalimi SM, Hua S, Weiler JE, Julian RJ: Myocardial Ca-sequestration failure and compensatory increase in Ca-ATPase with congestive cardiomyopathy: kinetic characterization by a homogenate microassay using real-time ratiometric indo-1 spectrofluorometry. Mol Cell Biochem 102: 1–12, 1991

    PubMed  Google Scholar 

  23. Ianuzzo CD, O'Brien PJ, Salerno TA, Laughlin MH: Effects of chronic tachycardia on the myocardium. In: GF Fletcher (ed.), Cardiovascular Response to Exercise. Futura Publishing Co Inc, Mount Kisco NY, pp 111–140, 1994

    Google Scholar 

  24. Ponce-Hornos JE: Energetics of calcium movements. In: GA Langer (ed.), Calcium and the Heart, Raven Press, Ltd, New York, NY, pp 269–298, 1990

    Google Scholar 

  25. Hasenfuss G, Mulieri LA, Leavitt BJ, Allen PD, Haeberle JR, Alpert NR: Alteration of contractile function and excitation-contraction coupling in dilated cardiomyopathy. Circ Res 70: 1225–1232, 1992

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    Peter James O'Brien & Hua Shen

  2. Department of Medicine, University of Toronto, M5S 1A8, Toronto, Ontario, Canada

    David Bissonette & Khursheed N. Jeejeebhoy

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  1. Peter James O'Brien
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  2. Hua Shen
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  3. David Bissonette
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  4. Khursheed N. Jeejeebhoy
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O'Brien, P.J., Shen, H., Bissonette, D. et al. Effects of hypocaloric feeding and refeeding on myocardial Ca and ATP cycling in the rat. Mol Cell Biochem 142, 151–161 (1995). https://doi.org/10.1007/BF00928936

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  • DOI: https://doi.org/10.1007/BF00928936

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