Abstract
Preparation and characterization of a monoclonal antibody termed M206 directed to a human monocyte lineage are described. The antibody was produced by somatic cell hybridization between BALB/c spleen cells primed with human histiocytic lymphoma cell line U937 and murine myeloma cell line P3U1. The antibody reacted intensely with human peripheral blood monocytes as well as U937 but did not react with peripheral blood T and B cells. Granulocytes were weakly stained with the antibody by indirect immunofluorescence. M206 also reacted intensely with the immature leukemic cells from patients with acute monocytic leukemia but was unreactive with other types of leukemic cells except cells from chronic myelogenous leukemia which showed varied patterns of reactivities. M206 reacted with a single polypeptide chain with a molecular weight of 180,000 on the surface of U937 cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bergholtz BO, Thorsby E: Macrophage-dependent response of immune human T lymphocytes to PPD in vitro. Scand J Immunol 6:779–786, 1977
Alter BJ, Bach FH: Lymphocyte reactivity in vitro. I. Cellular reconstruction of purified lymphocyte response. Cell Immunol 1:207–218, 1970
Rode HN, Gordon J: The mixed leukocyte culture: A three component system. J Immunol 104:1453–1457, 1970
Berlinger NT, Lopez Q, Good RA: Facilitation or attenuation of mixed leukocyte culture responsiveness by adherent cell. Nature 260:145–146, 1976
Rosenstreich DL, Mizel SB: The participation of macrophage cell lines in the activation of T lymphocytes by mitogens. Immunol Rev 40:102–135, 1978
Schmidtke JR, Hatfield S: Activation of purified human thymus derived cells by mitogens. II. Monocyte-macrophage potentiation of mitogen-induced DNA synthesis. J Immunol 116:357–362, 1976
Rosenberg SA, Lipsky PE: Monocyte dependence of pokeweed mitogen-induced differentiation of immunoglobulin-secreting cells from human peripheral blood monouclear cells. J Immunol 122:926–931, 1979
Nathan CF, Murray HW, Cohn ZA: The macrophage as an effector cells. N Engl J Med 303:622–626, 1980
Maizel AL, Metha SR, Ford RJ, Lachman LB: Effect of interleukin I on human thymocytes and purified human T cells. J Exp Med 153:470–475, 1981
Aardenet al.: Revised nomenclature for antigen-nonspecific T cell proliferation and helper factors. J Immunol 123:2928–2929, 1979
Ugolini V, Nunez G, Smith RG, Stastny P, Capra JD: Initial characterization of monoclonal antibodies against human monocytes. Proc Natl Acad Sci USA 77:6764–6768, 1980
Todd RF III, Nadler LM, Schlossman SF: Antigens on human monocytes identified by monoclonal antibodies. J Immunol 126:1435–1442, 1981
Raff HV, Picker LJ, Stobo JD: Macrophage heterogeneity in man. A subpopulation of HLA-DR bearing macrophages required for antigen induced T-cell activation also contains stimulators for autologous reactive T-cells. J Exp Med 152:581–593, 1980
Breard J, Reinherz EL, Kung PC, Goldstein G, Schlossman SF: A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol 124:1943–1948, 1980
Sundström C, Nilsson K: Establishment and characterization of a human histiocytic lymphoma cell line (U937). Int J Cancer 17:565–577, 1976
Koren HS, Anderson SJ, Larrick JW: In vitro activation of a human macrophage-like cell line. Nature 279:328–330, 1979
Larrick JW, Fischer DG, Anderson SJ, Koren HS: Characterization of a human macrophage-like cell line stimulated in vitro: A model of macrophage functions. J Immunol 125:6–12, 1980
Jondal M, Holm G, Wigzell H: Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med 136:207–215, 1972
Lachman LB, Moore JO, Metzgar RS: Preparation and characterization of lymphocyte-activating factor (LAF) from acute monocytic and myelomonocytic leukemia cells. Cell Immunol 41:199–206, 1978
Haustein D, Marchalonis JJ, Harris AW: Immunoglobulin of T lymphoma cells. Biosynthesis, surface representation and partial characterization. Biochem 14:1826–1834, 1975
Cone RE, Rosenstein RW: Isolation of T cell membrane proteins (IgT) with antisera to non-isotypic determinants of immunoglobulins: Evidence that IgT “Light” chains are not identical to B cell kappa chains. Mol Immunol 18:67–77, 1981
Kenett R, Mckearn TJ, Bechtol KB (eds): Monoclonal Antibodies. New York, Plenum Press, 1981, p 396
Dickler HB: Studies of the human lymphocyte receptor for heat-aggregated or antigen complexed immunoglobulin. J Exp Med 140:508–522, 1974
Cline MJ, Golde DW: Controlling the production of blood cells. Blood 53:157–164, 1979
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Maruyama, S., Naito, T., Kakita, H. et al. Preparation of a monoclonal antibody against human monocyte lineage. J Clin Immunol 3, 57–64 (1983). https://doi.org/10.1007/BF00919139
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00919139