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Impression cytology with transfer in xerophtalmia and conjunctival diseases

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Abstract

During a countrywide survey, we assessed the prevalence of clinical signs of xerophthalmia and of major conjunctival diseases in a randomized sample of 2,445 subjects representative of the population of the Republic of Djibouti. On a part of this sample, conjunctival Impression Cytology with Transfer (ICT) test and a plasma retinol determination were performed. Xerophthalmia as a public health problem was displayed by clinical signs (Bitot's spots, corneal scars among preschool children), low plasma retinol levels and ICT test results: 9.3% with deficient cytology in the rural area and 12.3% in the urban one (agestandardized rates). Results of ICT were related to age (p < 0.00001). Vitamin A deficiency was prevalent not only in preschool children but also up to 15 years. Moreover, ICT results are influenced by conjunctival diseases: compared to age-matched controls, there were more abnormal cytologies among patients with trachomatous inflammation (p=0.025), conjunctivitis (p=0.024) or Limbal Vernal Keratoconjunctivitis (p=0.015). Thus ICT shouldn't be performed among children with conjunctival diseases. In the region under study conjunctival diseases had high rates of prevalence: 16.4% of trachomatous scarrings in the urban area (standardized rate), 8% of conjunctivitis among rural preschool children, and 5% of Limbal Vernal Keratoconjunctivitis among children between 5 and 14 years in both areas.

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References

  1. Sporn MB, Robert AB, Goodman DW. The retinoids. Academic Press Inc., 1984, Vols 1–2.

  2. Sommer A, Hussaini G, Tarwotjo I, Susanto D. Increased mortality in children with mild vitamin A deficiency. Lancet 1983; 2: 585–588.

    Google Scholar 

  3. Sommer A, Tarwotjo I, Katz J. Increased risk of xerophthalmia following diarrhea and respiratory disease. Am J Clin Nutr 1987; 45: 977–980.

    Google Scholar 

  4. Egbert P, Lauber S, Maurice D. A simple conjunctival biopsy. Am J Ophthalmol 1984; 84: 798–801.

    Google Scholar 

  5. Amedee-Manesme O, Luzeau R, Carlier C, Ellrodt A. Simple impression cytology method for detecting vitamin A deficiency. Lancet 1987; 8544: 1263.

    Google Scholar 

  6. Amedee-Manesme O, Luzeau R, Wittpenn JR, Hanck A, Sommer A. Impression cytology detects subclinical vitamin A deficiency. Am J Clin Nutr 1988; 47: 875–878.

    Google Scholar 

  7. Luzeau R, Carlier C, Ellrodt A, Amedee-Manesme O. Impression cytology with transfer: an easy method for detection of vitamin A deficiency. Intern J Vit Nutr Res 1988; 58 (2): 166–170.

    Google Scholar 

  8. Cochran WC. Sampling techniques. New York: Wiley, 1963.

    Google Scholar 

  9. Henderson RH, Sundaresan T. Cluster sampling to assess immunization coverage: a review of experience with a simplified sampling method. Bulletin of the World Health Organization, 1982; 60 (2): 253–260.

    Google Scholar 

  10. Dawson CR, Jones BR, Tarizzo ML. Guide to trachoma control in programmes for the prevention of blindness. Geneva: World Health Organization, 1981.

    Google Scholar 

  11. Thylefors B, Dawson CR, Jones BR, West SK, Taylor HR. A simple system for the assessment of trachoma and its complications. Bulletin of the World Health Organization 1987; 65 (4): 477–483.

    Google Scholar 

  12. Sommer A. Field guide to the detection and control of xerophthalmia. 2nd ed. Geneva: World Health Organization, 1982.

    Google Scholar 

  13. Resnikoff S, Cornand G. Limbal Vernal Keratoconjunctivitis. Rev Int Trach 1988; 3–4: 53–72.

    Google Scholar 

  14. Catignani GL, Bieri JG. Simultaneous determination of retinol and tocopherol in serum or plasma by liquid chromatography. Clin Chem 1983; 29: 439–443.

    Google Scholar 

  15. WHO, UNICEF, USAID, HKI, IVACG. Control of vitamin A deficiency and xerophthalmia. Tech Rep Series 672. Geneva: World Health Organization, 1982.

    Google Scholar 

  16. Bauernfeind JC. Carotenoid vitamin A precursors and analogs in foods and feeds. J Agric Food Chem 1972; 20: 456–473.

    Google Scholar 

  17. Sommer A, Hussaini G, Muhilal H, Tarwotjo I, Susanto D, Saroso S. History of nightblindness: a simple tool for xerophthalmia screening. Am J Clin Nutr 1980; 33: 887–891.

    Google Scholar 

  18. Resnikoff S. Epidemiologic aspects of xerophthalmia in Chad. Med Trop 1988; 48 (1): 27–32.

    Google Scholar 

  19. Carlier C, Mouliat-Pelat JP, Ceccon JF, N'Diaye AM, Amedee-Manesme O. Prévalence du déficit en Vitamine A au Sénégal. GERM; in press.

  20. Resnikoff S, Filliard G, Dell'Aquila B, Abatte EA. Trachoma in Djibouti. Rev Int Trach 1989; 3–4: 129–142.

    Google Scholar 

  21. Amedee-Manesme O, De Meyer E. Stratégies diagnostiques et thérapeutiques dans la lutte contre le déficit en Vitamine A. Paris: INSERM-ORSTOM, 1989.

    Google Scholar 

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Resnikoff, S., Luzeau, R., Filliard, G. et al. Impression cytology with transfer in xerophtalmia and conjunctival diseases. Int Ophthalmol 16, 445–451 (1992). https://doi.org/10.1007/BF00918435

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  • DOI: https://doi.org/10.1007/BF00918435

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