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Hyperoxia stimulates alveolar macrophages to produce and release a factor which increases neutrophil adherence

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Abstract

Hyperoxia stimulates alveolar macrophages (AM) to make and release a factor which increases neutrophil adherence to nylon fiber. Production of the neutrophil adherence-stimulating factor by AM exposed to hyperoxia is maximal after AM have been exposed to hyperoxia for 72 h and requires protein synthesis by intact AM. The adherence factor is heat-labile and by column chromatography elutes in a molecular-weight range of approximately 8000–18,000 daltons. The AM-derived adherence factor could influence accumulation of neutrophils in the lungs of animals exposed to hyperoxia and contribute to neutrophil-mediated lung injury from hyperoxia.

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References

  1. Crapo, J. D., B. E. Barry, H. A. Foscue, andJ. Shelburne. 1980. Structural and biochemical changes in rat lungs occurring during exposures to lethal and adaptive doses of oxygen.Am. Rev. Respir. Dis. 122:123.

    Google Scholar 

  2. Shasby, D. M., R. B. Fox, R. N. Harada, andJ. E. Repine. 1982. Reduction of the edema of acute hyperoxic lung injury by granulocyte depletion.J. Appl. Physiol.52:1237.

    Google Scholar 

  3. Shasby, D. M., S. S. Shasby, andM. J. Peach. 1983. Granulocytes and phorbol myristate acetate increase permeability to albumin of cultured endothelial monolayers and isolated perfused lungs.Am. Rev. Respir. Dis. 127:72.

    Google Scholar 

  4. Shasby, D. M., K. M. VanBenthuysen, R. M. Tate, S. S. Shasby, I. F. McMurtry, andJ. E. Repine. 1982. Granulocytes mediate acute edematous lung injury in rabbits and isolated rabbit lungs perfused with phorbol myristate acetate: Role of oxygen radicals.Am. Rev. Respir. Dis. 125:443.

    Google Scholar 

  5. Kazmierowski, J. A., J. I. Gallin, andH. Y. Reynolds. 1977. Mechanism for the inflammatory response in primate lungs: Demonstration and partial characterization of an alveolar macrophage derived chemotactic factor with preferential activity for polymorphonuclear leukocytes.J. Clin. Invest. 59:273.

    Google Scholar 

  6. Hunninghake, G. W., J. I. Gallin, andA. S. Fauci. 1978. Immunologic reactivity of the lung: The in vivo and in vitro generaton of a neutrophil chemotactic factor by alveolar macrophages.Am. Rev. Respir. Dis. 117:15.

    Google Scholar 

  7. Merrill, W. W., G. P. Naegel, R. A. Matthay, andH. Y. Reynolds. 1980. Alveolar macrophage-derived chemotactic factor: Kinetics of in vitro production and partial characterization.J. Clin. Invest. 65:268.

    Google Scholar 

  8. Fox, R. B., J. R. Hoidal, D. M. Brown, andJ. E. Repine. 1981. Pulmonary inflammation due to oxygen toxicity: Involvement of chemotactic factors and polymorphonuclear leukocytes.Am. Rev. Respir. Dis. 123:521.

    Google Scholar 

  9. Harada, R. N., C. M. Bowman, R. B. Fox, andJ. E. Repine. 1982. Alveolar macrophage secretions: Initiators of inflammation in pulmonary oxygen toxicity?Chest 81:52.

    Google Scholar 

  10. Hoidal, J. R., G. D. Beall, F. L. Rasp, B. Holmes, J. G. White, andJ. E. Repine. 1978. Comparison of the metabolism of alveolar macrophages from humans, rats and rabbits: Response to heat-killed bacteria or phorbol myristate acetate.J. Lab. Clin. Med. 92:787.

    Google Scholar 

  11. Repine, J. E., J. G. White, C. C. Clawson, andB. M. Holmes. 1974. The effect of phorbol myristate acetate on the metabolism and ultrastructure of neutrophils in chronic granulomatous disease.J. Clin. Invest. 54:83.

    Google Scholar 

  12. MacGregor, R. R., P. J. Spagnulo, andA. L. Lentnek. 1974. Inhibition of granulocyte adherence by ethanol, prednisolone, and aspirin, measured with an assay system.N. Engl. J. Med. 291:642.

    Google Scholar 

  13. Rasp, F. L., C. C. Clawson, J. R. Hoidal, andJ. E. Repine. 1979. Quantitation and scanning electron microscopic comparison of human alveolar macrophage and polymorphonuclear leukocyte adherence to nylon fibers in vitro.J. Reticuloendothel. Soc. 25:101.

    Google Scholar 

  14. Repine, J. E., andC. C. Clawson. 1978. Influence of surface proteins and separation techniques on neutrophil unstimulated and stimulated locomotion in vitro.J. Reticuloendothel. Soc. 24:217.

    Google Scholar 

  15. Hoidal, J. R., R. B. Fox, andJ. E. Repine. 1979. Defective oxidative metabolic responses in vitro of alveolar macrophages in chronic granulomatous disease.Am. Rev. Respir. Dis. 120:613.

    Google Scholar 

  16. Nathan, C. F., H. W. Murray, andZ. A. Cohn. 1980. Current concepts: The macrophages as an effector cell.N. Engl. J. Med. 303:622.

    Google Scholar 

  17. Boxer, L. A., M. Yoder, S. Bonsib, M. Schmidt, P. Ho, R. Jersilo, andR. L. Baehner. 1979. Effects of a chemotactic factor, N-formyl methionyl peptide, on adherence, superoxide anion generation, phagocytosis, and microtubule assembly of human polymorphonuclear leukocytes.J. Lab. Clin. Med. 93:506.

    Google Scholar 

  18. O'Flaherty, J. T., D. L. Kreutzer, andP. A. Ward. 1978. Chemotactic factor influences on the aggregation, swelling, and foreign surface adhesiveness of human leukocytes.Am. J. Pathol. 90:537.

    Google Scholar 

  19. Repine, J. E., C. M. Bowman, andR. M. Tate. 1982. Neutrophils and the lung. State of the art.Chest 81:47.

    Google Scholar 

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Authors and Affiliations

  1. Webb-Waring Lung Institute and Departments of Pediatrics and Medicine, School of Medicine University of Colorado Health Sciences Center, 80262, Denver, Colorado

    C. Michael Bowman, Ruth N. Harada & John E. Repine

Authors
  1. C. Michael Bowman
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  2. Ruth N. Harada
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  3. John E. Repine
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Additional information

This work was supported by grants from the Americans Heart Association, National Institutes of Health HL24248, Council for Tobacco Research, and the Kroc. hill, Swan, Kleberg and Parker B. Francis Foundation.

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Bowman, C.M., Harada, R.N. & Repine, J.E. Hyperoxia stimulates alveolar macrophages to produce and release a factor which increases neutrophil adherence. Inflammation 7, 331–338 (1983). https://doi.org/10.1007/BF00916297

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