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Involvement of inflammatory leukocytes in hepatic induction of T-kininogen in rat

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Abstract

Hepatic synthesis as well as plasma levels of T-kininogen, a protein precursor of T-kinin (Ile-Ser-bradykinin), increase in rats following the induction of acute inflammation. Studies have been undertaken to elucidate an involvement of inflammatory leukocytes in the acute-phase response of T-kininogen. Peritoneal exudate cells (PEC) were prepared from rats six days after the intraperitoneal injection of Freund's complete adjuvant. By transfer of these leukocytes into the peritoneal cavity of normal rats, plasma kininogen levels of these recipients increased about threefold after one day. Secretion of kininogen from rat hepatocytes in primary culture was enhanced about twofold by coculturing with PEC. A similar effect was also obtained by adding culture supernatant of these leukocytes into hepatocytes, and the increased levels of kininogen in culture medium of hepatocytes was due to the increased levels of T-kininogen. From these results, it was concluded that leukocytes in the inflammatory site, probably macrophages, release some substance(s) which stimulate(s) the hepatic synthesis of T-kininogen.

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References

  1. Kato, H., S., Nagasawa, andS. Iwanaga. 1981. HMW and LMW kininogens.Methods Enzymol. 80:172.

    Google Scholar 

  2. Okamoto, H., andL. M. Greenbaum. 1983. Kininogen substrates for trypsin and cathepsin D in human, rabbit and rat plasma.Life Sci. 32:2007.

    Google Scholar 

  3. Okamoto, H., andL. M. Greenbaum. 1983. Isolation and structure of T-kinin.Biochem. Biophys. Res. Commun. 112:701.

    Google Scholar 

  4. Zach, H. P., andE. Werle. 1972. Changes in kininogen content of serum and some organs during injury and inflammation in rats.Adv. Exp. Med. Biol. 21:371.

    Google Scholar 

  5. Borges, D. R. andA. H. Gordon. 1976. Kininogen and kininogenase synthesis by the liver of normal and injured rats.J. Pharm. Pharmacol. 28:44.

    Google Scholar 

  6. Barlas, A., H. Okamoto, andL. M. Greenbaum. 1985. T-Kininogen—the major plasma kininogen in rat adjuvant arthritis.Biochem. Biophys. Res. Commun. 129:280.

    Google Scholar 

  7. Kageyama, R., N. Kitamura, H. Ohkubo, andS. Nakanishi. 1985. Differential expression of the multiple forms of rat prekininogen mRNA after acute inflammation.J. Biol. Chem. 260:12060.

    Google Scholar 

  8. Barlas, A., K. Sugio, andL. M. Greenbaum. 1985. Release of T-kinin and bradykinin in carrageenin induced inflammation in the rat.FEBS Lett. 190:268.

    Google Scholar 

  9. Kushner, I. 1982. The phenomenon of the acute-phase response.Ann. N. Y. Acad. Sci. 389:39.

    Google Scholar 

  10. Rupp, R. G., andG. M. Fuller. 1979. The effects of leukocytic and serum factors on fibrinogen biosynthesis in cultured hepatocytes,Exp. Cell Res. 118:23.

    Google Scholar 

  11. Woloski, B. M. R. N. J., E. Gospodarek, andJ. C. Jamieson. 1985. Studies on monokines as mediators of the acute phase response: Effect on sialyltransferase, albumin,α 1-acid glycoprotein andβ-N-acetylhexosamidase.Biochem. Biophys. Res. Commun. 130:30.

    Google Scholar 

  12. Woloski, B. M. R. N. J., andG. M. Fuller. 1985. Identification and partial characterization of hepatocyte-stimulating factor from leukemia cell lines: Comparison with interleukin I.Proc. Natl. Acad. Sci. U.S.A. 82:1443.

    Google Scholar 

  13. Yoshinaga, M., S. Nakamura, andH. Hayashi. 1975. Interaction between lymphocytes and inflammatory exudate cells.J. Immunol. 115:533.

    Google Scholar 

  14. Yoshinaga, M., A. Yoshinaga, andB. H. Waksman. 1972. Regulation of lymphocyte responses in vitro. I. Regulatory effect of macrophages and thymus-dependent (T) cells on the response of thymus-independent (B) lymphocytes to endotoxin.J. Exp. Med. 136:956.

    Google Scholar 

  15. Seglen, P. O. 1973. Preparation of rat liver cells. III. Enzymatic requirements for tissue dispersion.Exp. Cell Res. 82:391.

    Google Scholar 

  16. Salvesen, G., C. Parkes, M. Abrahamson, A. Grubb, andA. J. Barrett. 1986. Human low-Mr kininogen contains three copies of a cystatin sequence that are divergent in structure and in inhibitory activity for cysteine proteinases.Biochem. J. 234:429.

    Google Scholar 

  17. Barrett, A. J. 1981. Cystatin: The egg white inhibitor of cysteine proteinases.Methods Enzymol. 80:771.

    Google Scholar 

  18. Odya, C. E., T. L. Goodfriend, J. M. Stewart, andC. Pena. 1978. Aspects of bradykinin radioimmunoassay.J. Immunol. Methods 19:243.

    Google Scholar 

  19. Adams, D. O. 1982. Macrophage activation and secretion.Fed. Proc. 41:2193.

    Google Scholar 

  20. Kampschmidt, R. F., H. F. Upchurch, andL. A. Pulliam. 1982. Characterization of a leukocyte-derived endogeneous mediator responsible for increase plasma fibrinogen.Ann. N.Y. Acad. Sci. 389:338.

    Google Scholar 

  21. Oppenheim, J. J., B. M. Stadler, R. P. Siraganian, M. Mage, andB. Mathieson. 1982. Lymphokines: Their role in lymphocyte response—Properties of interleukin I.Fed. Proc. 41:257.

    Google Scholar 

  22. Bauer, J., W. Weber, T. Tran-Thi, G. Northoff, K. Decker, W. Gerok, andP. C. Heinrich. 1985. Murine interleukin I stimulates α2-macroglobulin synthesis in rat hepatocyte primary cultures.FEBS Lett. 190:271.

    Google Scholar 

  23. Ritchie, D. G., andG. M. Fuller. 1983. Hepatocyte-stimulating factor: A monocyte-derived acute-phase regulatory protein.Ann. N. Y. Acad. Sci. 408:490.

    Google Scholar 

  24. Okamoto, H., andL. M. Greenbaum. 1983. Pharmacological properties of T-kinin (isoleucyl-seryl-bradykinin) from rat plasma.Biochem. Pharmacol. 32:2637.

    Google Scholar 

  25. Sueyoshi, T., K. Eniyoji, T. Shimada, H. Kato, S. Iwanaga, Y. Bando, E. Kominami, andN. Katunuma. 1985. A new function of kininogens as thiol-proteinase inhibitors: Inhibition of papain and cathepsin B, H, and L by bovine, rat, and human plasma kininogens.FEBS Lett. 182:193.

    Google Scholar 

  26. Barrett, A. J., H. Fritz, A. Grubb, S. Isemura, M. Jarvinen, N. Katunuma, W. Nachleidt, W. Muller-Esterl, M. Sasaki, andV. Turk. 1986. Nomenclature and classification of the proteins homologous with the cysteine-proteinase inhibitor chicken cystatin.Biochem. J. 236:312.

    Google Scholar 

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This study was supported in part by a Grant-in-Aid from the Naito Foundation and one (No. 61580154) from the Ministry of Education, Science and Culture of Japan.

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Itoh, N., Toyohama, T., Okamoto, H. et al. Involvement of inflammatory leukocytes in hepatic induction of T-kininogen in rat. Inflammation 11, 345–352 (1987). https://doi.org/10.1007/BF00915838

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