Abstract
The specificity and kinetics of hyaluronic acid (HA) accumulation in relation to other glycosaminoglycans (GASs) were determined in rabbit lungs during an allergic granulomatous response to BCG, an allergic nongranulomatous response to tuberculoprotein, and during a foreign-body granulomatous response to carrageenan. Hyaluronic acid was the only GAG detected in the lung lavage fluids. Hyaluronic acid occurred in the airways on day two of the allergic granulomatous response, but its presence in the airway did not correlate with ensuing granuloma formation in the parenchyma. Generalized increases in GAG of the parenchyma also peaked on day two of the DTH responses. Generalized increases in GAG peaked on day five during the foreign-body granulomatous response to carrageenan. A persistently elevated level of HA in the lung tissue correlated with granuloma formation but not with the intensity of the response.
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References
Galindo, B., Q. N. Myrvik, andS. H. Love. 1975. A macrophage agglutinating factor produced during a pulmonary delayed hypersensitivity reaction.J. Reticuloendothel. Soc. 18:295–304.
Love, S. H., B. T. Shannon, Q. N. Myrvik, andW. S. Lynn. 1979. Characterization of macrophage agglutinating factor as a hyaluronic acid-protein complex.J. Reticuloendothel. Soc. 25:269–282.
Wusteman, F. S. 1972. Glycosaminoglycans of bovine lung parenchyma and pleura.Experientia 28:887–888.
Horwitz, A. L., andR. G. Crystal. 1975. Content and synthesis of glycosaminoglycans in the developing lung.J. Clin. Invest. 56:1312–1318.
Sahu, S., andW. S. Lynn. 1978. Hyaluronic acid in the pulmonary secretions of patients with asthma.Biochem. J. 173:565–568.
Sahu, S., andW. S. Lynn. 1978. Hyaluronic acid in the pulmonary secretions of patients with alveolar proteinosis.Inflammation 3:149–158.
Havez, R., P. Degand, A. Boersma, andC. Richet. 1971. Characterization et dosage de l acide hyaluronique dans le liquide pleural de mesotheliome.Clin. Chim. Acta 33:443–454.
McGee, M. P., Q. N. Myrvik, andE. S. Leake. 1978. Organization of allergic granulomas and dependence on insoluble antigen.J. Reticuloendothel. Soc. 24:253–262.
Warburg, O., andW. Christian. 1942. Isolation and crystallization of enolase.Biochem. Z. 310:384–421.
Bragdon, J. H. 1951. Colorimetric determination of blood lipids.J. Biol. Chem. 190: 513–517.
Lowry, O. H., N. J. Rosebrough, A. L. Farr, andR. J. Randall. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265–275.
Blumenkrantz, N., andG. Asboe-Hansen. 1973. New method for quantitative determination of uronic acids.Anal. Biochem. 54:484–489.
Blumenkrantz, N., andG. Asboe-Hanson. 1976. An assay for total hexosamine and a differential assay for glucosamine and galactosamine.Clin. Biochem. 9:269–174.
Hata, R., andY. Nagai. 1972. A rapid and micro method for separation of acidic glycosaminoglycans by two-dimensional electrophresis.Anal. Biochem. 45:462–468.
Hata, R., andY. Nagai. 1973. A micro colorimetric determination of acidic glycosaminoglycans by two dimensional electrophoresis on a cellulose acetate strip.Anal. Biochem. 52:652–656.
Saito, H., T. Yamagata, andS. Suzuki. 1968. Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates.J. Biol. Chem. 243:1523–1535.
Stevens, R. L., M. Colombo, J. J. Gonzales, W. Hollander, andK. Schmid. 1976. The glycosaminoglycans of the human artery and their changes in atherosclerosis.J. Clin. Invest. 58:470–481.
Roden, L., J. R. Baker, J. A. Cifonelli, andM. B. Mathews. 1972. Isolation and characterization of connective tissue polysaccharides.Methods Enzymol., Vol.28:73–140.
Zar, J. H. 1974. Biostatistical Analysis. Prentice-Hall, Englewood Cliffs, New Jersey.
Velo, G. P., andW. G. Spector. 1973. The origin and turnover of alveolar macrophages in experimental pneumonia.J. Pathol. 109:7–19.
Peterson, L. B., R. S. Thrall, V. L. Moore, J. O. Stevens, andP. Abramoff. 1977. An animal model of hypersensitivity pneumonitis in the rabbit. Induction of cellular hypersensitivity to inhaled antigens using carrageenan and BCG.Am. Rev. Respir. Dis. 116: 1007–1012.
Shannon, B. T., S. H. Love, andQ. N. Myrvik. 1980. Participation of hyaluronic acid in the macrophage disappearance reaction.Immunol. Commun. 9:357–370.
Greiling, H., W. Mohr, andG. Beneke. 1972. Nachweis and bestimmungeines hyaluronatprotein-komplexes in der peritonealflossigkeit von ratten nach injektion von phytohamagglutinin.Z. Naturforsch. (B) 27:414–419.
Dove, F., A. Hale, andC. Underbill. 1979. Rabbit normal alveolar macrophages (AM) bind tritiated hyaluronic acid (3H-HA) in amounts which reflect their rate of agglutination by hyaluronic acid (HA).J. Reticuloendothel. Soc. 26(Suppl.):27a.
Goggins, J. F., G. S. Lazarus, andH. M. Fullmer. 1968. Hyaluronidase activity of alveolar macrophages.J. Histochem. Cytochem. 16:688–692.
Galindo, B. 1972. Antigen-mediated fusion of specifically sensitized rabbit alveolar macrophages.Infect. Immunol. 5:583–594.
Warfel, A. H. 1978. Macrophage fusion and multinucleated giant cell formation, surface morphology.Exp. Mol. Pathol. 28:163–176.
Skinses, O. K., andE. Matsuo. 1974. Acid mucopolysaccharide metabolism in leprosy. 1. Storage of hyaluronic acid and its possible significance in the pathogenesis of leprosy.Int. J. Lepr. 42:392–398.
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Shannon, B.T., Love, S.H., Roh, B.H. et al. Quantitation of glycosaminoglycans of rabbit lung during delayed-type hypersensitivity reactions and granuloma formation. Inflammation 5, 323–334 (1981). https://doi.org/10.1007/BF00911096
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DOI: https://doi.org/10.1007/BF00911096