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Enhancement of [123I]β-CIT binding in the striatum with clomipramine: Is there a serotonin-dopamine interaction?

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Abstract

Many reports support the concept of serotonergic-dopaminergic interaction in the brain. However, at present, there are few methods to study this relationship in vivo. The purpose of this study was to investigate the effect of serotonin (5-HT) uptake inhibitor, clomipramine, on a dopamine (DA) transporter ligand, [123I]β-CIT (RTI-55), in rat brain. Dose-dependent changes in [123I]β-CIT specific binding induced by clomipramine were studied in the striatum (rich in DA transporter) and the hypothalamus (rich in 5-HT transporter). The changes in the time-activity curves of [123I]β-CIT specific binding after clomipramine injection were also examined in these two regions. Using the cerebellum as the reference region,k 3 andk 4 values with and without clomipramine administration were estimated by a two-compartment kinetic analysis. Clomipramine inhibited [123I]β-CIT specific binding in the hypothalamus, but enhanced its specific binding in the striatum in a dose-dependent manner. Kinetic analysis showed thatk 3 in the striatum was increased by 55%. In conclusion, enhancement of [123I]β-CIT binding in the striatum after clomipramine administration indicated the possibility of 5-HT-DA interaction.

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Authors and Affiliations

  1. Division of Tracer Kinectics, Biomedical Research Center, Osaka University Medical School, Osaka 565, Japan

    Masahiro Fujita & Tsunehiko Nishimura

  2. Daiichi Radioisotope Laboratories, Ltd., 289-15, Chiba, Japan

    Keizo Takatoku, Yoshinori Matoba & Masaru Nishiura

  3. Department of Medical Physics, School of Allied Health Science, Faculty of Medicine, Osaka University, Osaka 565, Japan

    Kaoru Kobayashi & Osamu Inoue

  4. Department of Psychiatry, Yale University School of Medicine, VA Medical Center, 06516, West Haven, CT, USA

    Masahiro Fujita

Authors
  1. Masahiro Fujita
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  2. Keizo Takatoku
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  3. Yoshinori Matoba
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  4. Masaru Nishiura
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  5. Kaoru Kobayashi
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  6. Tsunehiko Nishimura
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  7. Osamu Inoue
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Fujita, M., Takatoku, K., Matoba, Y. et al. Enhancement of [123I]β-CIT binding in the striatum with clomipramine: Is there a serotonin-dopamine interaction?. Eur J Nucl Med 24, 403–408 (1997). https://doi.org/10.1007/BF00881812

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  • DOI: https://doi.org/10.1007/BF00881812

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