Summary
Electrophoretic separation of hemoglobins of normalXenopus laevis and of isogenic animals derived from female hybrids ofXenopus laevis×Xenopus gilli revealed 5–9 components in premetamorphic larvae, and 3–4 components in adult toads. InXenopus laevis the number of larval hemoglobin components showed considerable variation, but this variation was absent in isogenic tadpoles, suggesting a genetic basis for hemoglobin polymorphism in larvae.
Electrophoretic separation of larval and adult hemoglobins at different concentrations of acrylamide and treatment of these solutions with mercaptoethanol revealed that larval hemoglobin components are charge isomers, whereas adult hemoglobin was found to contain a minor dimeric component.
Estimation of hemoglobin components showed that the main increase in adult hemoglobin, i.e from 30–90% of total hemoglobin, occurs within 4 weeks after completion of metamorphosis. By incroporation of3H amino acids in vivo a switch to preferential synthesis of adult hemoglobin and a corresponding decrease in larval hemoglobin production could be demonstrated during early climax stages. This suggests that thyroid hormones are involved in the hemoglobin transition. Yet chemical inhibition of the larval thyroid by thiourea resulted in a delayed but complete hemoglobin transition without morphological transformation. It is concluded that hemoglobin transition and morphological transformation of theXenopus tadpole require different concentrations of thyroid hormones.
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Abbreviations
- Hb:
-
hemoglobin
- HbA :
-
adult hemoglobin
- HbL :
-
larval hemoglobin
References
Battaglia, P., Melli, M.: Isolation of globin messenger RNA ofXenopus laevis. Develop. Biol.58, 337–350 (1977).
Bruns, G.A.P., Ingram, V.M.: The erythroid cells and hemoglobins of the chick embryo. Phil. Trans. Roy. Soc. London266B, 225–235 (1973)
Ducibella, T.: The occurrence of biochemical metamorphic events without anatomical metamorphosis in the axolotl. Develop. Biol.38, 175–186 (1974)
Frieden, E., Just, J.J.: Hormone responses in amphibian metamorphosis. In: Mechanisms of Hormone Action (G. Litwack, ed.), Vol. 1, pp. 1–52. New York Academic Press 1970
Gasche, P.: Zur Frage des Angriffspunktes des Thiouracil. Experientia2, 24–26 (1946)
Hedrick, J., Smith, A.: Size and charge isomer separation and estimation of molecular weight of proteins by disc gel electrophoresis. Arch Biochem. Biophys.126, 155–164 (1968).
Jurd, R.D., Maclean, N.: The investigation ofXenopus laevis haemoglobins during development by a fluorescent antibody. Experientia25, 626–628 (1969)
Jurd, R.D., Maclean, N.: An immunofluorescent study of the haemoglobins in metamorphosingXenopus laevis. J. Embryol. exp. Morph.23, 299–309 (1970)
Jurd, R.D., Maclean, N.: Detection of haemoglobin in red cells ofXenopus laevis by immunofluorescent double labelling. J. Microscopy100, 213–217 (1974)
Just, J.J., Atkinson, B.G.: Hemoglobin transition in the bullfrog,Rana catesbeiana, during spontaneous and induced metamorphosis. J. Exp. Zool.182, 271–280 (1972)
Kobel, H.R., Du Pasquier, L.: Production of large clones of histocompatible fully identical clawed toadsXenopus. Immunogenetics2, 87–91 (1975).
Kollros, J.J.: Mechanisms of amphibian metamorphosis: Hormonoes Am. Zoologist.1, 107–114 (1961)
Maclean, N., Brooks, G.T., Jurd, R.D.: Haemoglobin synthesisin vitro by erythrocytes fromXenopus laevis. Comp. Biochem. Physiol.30, 825–834 (1969)
Maclean, N., Jurd, R.D.: The haemoglobins of healthy and anaemicXenopus laevis. J. Cell Sci.9, 509–528 (1971)
Maclean, N., Jurd, R.D.: The control of haemoglobin synthesis. Biol. Rev.47, 393–437 (1972)
Maclean, N., Turner, S.: Adult haemoglobin in developmentally retarded tadpoles ofXenopus laevis. J. Embryol. exp. Morph.35, 261–266 (1976)
Moss, B., Ingram, V.M.: Hemoglobin synthesis during amphibian metamorphosis. I. Chemical studies on the hemoglobins from the larval and adult stages ofRana catesbeina. J. Mol. Biol.32, 481–492 (1968a)
Moss, B., Ingram, V.M.: Hemoglobin synthesis during amphibian metamorphosis. II. Synthesis of adult hemoglobin following thyroxine administration. J. Mol. Biol.32, 493–504 (1968b)
Nieuwkoop, P.D., Faber, J.: Normal Table ofXenopus laevis Daudin. North Holland Publishing Co., Amsterdam 1956
Pflugfelder, O.: Beeinflussung der Thyreoidea und anderer Organe des Haushuhnes durch Kaliumperchlorat. Mit vergleichenden Untersuchungen an niederen Wirbeltieren. Roux' Arch. Entwickl. Mech. Org.151, 78–112 (1959)
Saxén, L., Saxén, E., Toivonen, S., Salimaki, K.: Quantitative investigations into the anterior pituitary-thyroid mechanism during frog metamorphosis. Endocrinology61, 35–44 (1957)
Sullivan, B.: Amphibian hemoglobins In: Chemical Zoology (M. Florkin and B.T. Scheer, eds.), Vol. 9, pp. 77–122. New York: Academic Press 1974
Tompkins, R., Townsend, J.K.: Control of metamorphic events in a neotenous urodeleAmbystoma mexicanum. J. Exp. Zool.200, 191–196 (1977)
Wabl, M.R., Du Pasquier, L.: Antibody patterns in genetically identical frogs. Nature264, 642–644 (1976)
Weber, R.: Biochemistry of amphibian metamorphosis. In: The Biochemistry of Animal Development (R. Weber, ed.), Vol. 2, pp. 277–301, New York: Academic Press 1967
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Just, J.J., Schwager, J. & Weber, R. Hemoglobin transition in relation to metamorphosis in normal and isogenicXenopus . Wilhelm Roux' Archiv 183, 307–323 (1977). https://doi.org/10.1007/BF00848460
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DOI: https://doi.org/10.1007/BF00848460