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Nucleotides, coenzymes, phosphate esters. XXIV. Hydrolytic cleavage of thiamine triphosphate and production of cocarboxylase hydrochloride

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Conclusions

  1. 1.

    Thiamine triphosphate was hydrolyzed, followed by isolation of the thiamine diphosphate (cocarboxylase) formed on ion-exchange resins.

  2. 2.

    Cocarboxylase was obtained in the form of the hydrochloride; the possibility of freeing it from the admixture of ionogenic orthophosphoric acid by precipitation from an aqueous hydrochloric acid solution with a mixture of anhydrous alcohol with hydrochloric acid (10∶:0.8) or by crystallization from an acidified aqueous alcohol solution, was demonstrated; cocarboxylase was isolated in the form of a crystalline preparation with a high degree of purity.

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Authors and Affiliations

  1. All-Union Vitamin Scientific-Research Institute, Moscow

    V. M. Berezovskii, V. I. Koltunova & N. S. Genokhova

Authors
  1. V. M. Berezovskii
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  2. V. I. Koltunova
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  3. N. S. Genokhova
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Translated from Khimiko-Farmatsevticheskii Zhurnal, No. 8, pp. 40–45, August, 1969.

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Berezovskii, V.M., Koltunova, V.I. & Genokhova, N.S. Nucleotides, coenzymes, phosphate esters. XXIV. Hydrolytic cleavage of thiamine triphosphate and production of cocarboxylase hydrochloride. Pharm Chem J 3, 463–467 (1969). https://doi.org/10.1007/BF00833003

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  • DOI: https://doi.org/10.1007/BF00833003

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