Abstract
The properties of two polymers obtained from flagellin by different methods — precipitation by ammonium sulfate followed by centrifugation or treatment with glutaraldehyde in solution followed by gel chromatography — were studied. Molecules of the former (POL) and the latter (GLUT) are similar in shape but POL has a higher molecular weight. The preparations contain a common H antigen and are similar in serologic activity. POL has very high “priming” activity for mice. GLUT is highly immunogenic only within a narrow dose range, is less immunogenic than POL, and differs from POL in forming a certain quantity of 7S antibodies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
G. L. Ada, Nature (London),199, 1257 (1963).
J. Davie and W. Paul, J. Exp. Med.,135, 660 (1972).
M. Feldmann, J. Exp. Med.,135, 735 (1972).
G. Nossal, G. L. Ada, and C. Austin, Aust. J. Exp. Biol. Med. Sci.,42, 283 (1964).
C. R. Parish, J. Exp. Med.,134, 21 (1971).
R. Patterson, J. Suszko, and F. McIntire, J. Immunol.,110, 1402 (1973).
R. A. Schlegel, Aust. J. Exp. Biol. Med. Sci.,52, 99 (1974).
Rights and permissions
About this article
Cite this article
Lyashenko, V.L., Aleksander, S.K., Vaneeva, L.I. et al. Immunogenicity of polymers of H antigen obtained by different methods. Bull Exp Biol Med 85, 601–604 (1978). https://doi.org/10.1007/BF00806383
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00806383