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Immunochemical analysis of hemolymph clotting in the insectLeucophaea maderae (Blattaria)

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Summary

Hemolymph clotting in the Blattarian speciesLeucophaea maderae was analyzed by immunological methods.

  1. 1.

    Experimentally induced (by freezing and thawing) disintegration of hemocytes — in the absence of plasma proteins — is followed by the formation of a clot-like gel. This gel resists treatment with strong detergents, but is dissolved by reducing agents and proteolytic enzymes. The gel material forms part of the native hemolymph clot. It is mainly contained in the granules of the hemocytes from which it is released during clotting. This hemocyte derived clotting protein is calledhemocyte coagulogen.

  2. 2.

    During normal hemolymph clotting one of the plasma proteins disappears from the hemolymph; this protein also becomes incorporated into the native hemolymph clot which is — as compared to the gel — more rigid and not soluble in reducing agents. This second clotting protein — theplasma coagulogen —cannot be found in intact hemocytes.

  3. 3.

    Clotting of the plasma coagulogen is possible only in the presence of the hemocyte coagulogen.

  4. 4.

    Both coagulogens are shown to be present in theislands of coagulation at exactly the same places.

  5. 5.

    Conditions of plasma clotting, differences in solubility between gel and clot, and the common occurrence of both coagulogens in the islands of coagulation are strong arguments for an interaction of the two coagulogens during clotting; the hypothesis is put forward that the two coagulogens in the native clot are interconnected to form a common network.

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Bohn, H., Barwig, B. & Bohn, B. Immunochemical analysis of hemolymph clotting in the insectLeucophaea maderae (Blattaria). J Comp Physiol B 143, 169–184 (1981). https://doi.org/10.1007/BF00797696

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