Abstract
A culture vessel consisting of two independent chambers separated only by the growth substrate is described. Cells may be cultured on both sides of the growth substrate. Culture medium and gas exposure can independently be controlled in both compartments. Human hair follicles have been used as source of keratinocytes and the bovine eye lens capsule has been explored as growth substrate. The presence of 5% CO2 in air in the lower compartment appears to have a significant effect on the morphology of the cultures. When the cultures are being exposed to air with 5% CO2, the culture medium being applied in the lower compartment, formation of corneocytes characteristic for adult stratum corneum is induced, as evidenced by light and electron microscopy. To the knowledge of the authors, this stage of differentiationin vitro has not been obtained with previously described systems. Differentiation of the lower cell layers has been characterised with specific antibodies. The possible use of the system for applied and pure scientific research is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Asselineau, D. & Prunieras, M., 1981. British Journal of Dermatology 111: 219s-222s.
Bell, E., Ivarsson, B. & Merrill, C., 1979. Proceedings of the National Academy of Sciences, U.S.A. 76: 1274–1278.
Bernard, B. A., Robinson, S. M., Vandaele, S., Mansbridge, J. N. & Darmon, M., 1985. British Journal of Dermatology (In press).
Breathnach, A. S., 1971. In: An atlas of the ultrastructure of human skin. Development, differentiation and post-natal features, J. & A. Churchill, London, pp. 68–69.
Breathnach, A. S., 1971. In: An atlas of the ultrastructure of human skin. Development, differentiation and post-natal features. J. & A. Churchill, London, pp. 128–129.
Cairns, J., 1975. Nature 255: 197–200.
Fox, C. H., Selkirk, K., Price, F. M., Croy, R. G., Sanford, U. K. & Cottler-Fox, M., 1975. Cancer Research 35: 3551–3557.
Freeman, A. E., Igel, U. J., Herrman, B. J. & Kleinfeld, K. L., 1976.In vitro 12: 352–362.
Hukkelhoven, M. W. A. C., Vermorken, A. J. M. & Bloemendal, H., 1981. Preparative Biochemistry 10: 473–481.
Leighton, J., 1951. Journal of the National Cancer Institute 12: 545–561.
Leighton, J., 1954. Journal of the National Cancer Institute 15: 275–293.
Lenoir, M. C., Bernard, B. A., Ferracin, J., Shroot, B. & Vermorken, A. J. M. (Submitted for publication).
Prunieras, M., Régnicer, M. & Woodley, F., 1983. The Journal of Investigative Dermatology 81: 28s-33s.
Régnier, M., Prunieras, M. & Woodley, D., 1981. Frontiers in Matrix Biology 9: 4–35.
Shuster, S., 1984. British Journal of Dermatology 111: 235–242.
Vermorken, A. J. M. & Bloemendal, H., 1978. Nature 271: 779–781.
Vermorken, A. J. M., Lenoir, M. C., Shroot, B., Kimenai, P. M., Vermeesch-Markslag, A. M. G., 1984. Molecular Biology Reports 10: 41–47.
Vermorken, A. J. M. & Shroot, B., 1984. British Journal of Dermatology 111: 73s-82s.
Weterings, P. J. J. M., Vermorken, A. J. M. & Bloemendal, H., 1981. British Journal of Dermatology 104: 1–5.
Weterings, P. J. J. M., Vermorken, A. J. M. & Bloemendal, H., 1982. Experimental Cell Research 139: 439–443.
Weterings, P. J. J. M., Verhagen, H., Wirtz, P. & Vermorken, A. J. M., 1984. Virchows Archiv B. 45: 255–266.
Woodley, D., Didierjan, L., Régnier, M., Saurat, J. & Prunieras, M., 1980. The Journal of Investigative Dermatology 75: 148–151.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vermorken, A.J.M., Verhagen, H., Vermeesch-Markslag, A.M.G. et al. Differentiation of keratinocytesin vitro: a new culture vessel mimicking thein vivo situation. Mol Biol Rep 10, 205–213 (1985). https://doi.org/10.1007/BF00775977
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00775977