Abstract
The radius of gyration of mononucleosomes determined by X-ray small-angle scattering is 4.35 nm. The maximum dimension determined from the distance distribution function and the volume amount to 12.9 nm and 370 nm3, respectively. For a particular fraction of polynucleosomes a mean radius of gyration 16 nm, a maximum dimension 65 nm, and a mean volume 25,240 nm3 is obtained.The shape is approximated by an elongated cylinder having a diameter of 28 nm. A polynucleosome is built up from 69 nucleosomes, on the average. The distance of neighbouring nucleosomes in the polynucleosome amounts to 5.2 nm. Moreover, this distance shows that the nucleosomes in the polynucleosome are very closely packed.
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References
PardonJ.F., D.L.Worcester, J.C.Wooley et al. Nucl. Acids Res. 2: 2163–2176 (1975).
HjelmR.P., G.G.Kneale, P.Suau et al. Cell 10: 139–151 (1977).
SuauP., G.G.Kneale, G.W.Braddock et al. Nucl. Acids Res. 4: 3769–3786 (1977).
PardonJ.F., R.I.Cotter, D.M.J.Lilley et al. Cold Spring Harbor Symp. Quant. Biol. 42: 11–22 (1978).
KnealeG.G., J.P.Baldwin & E.M.Bradbury. Quarterly Reviews of Biophysics 10: 485–527 (1977).
Damaschun,H., G. Damaschun, H. Fenske et al. Acta biol. med. germ. K13–K17 (1977).
Chromatin, Cold Spring Harbor Symp. Quant. Biol. 42 (1978).
AleksanyanV.J., V.I.Vorob'ev & B.A.Fedorov. Biopolymers 14: 1133–1142 (1975).
SperlingL. & A.Tardieu. FEBS letters 64: 89–91 (1976).
CampbellA.M., R.J.Cotter & J.F.Pardon. Nucl. Acids Res. 5: 1571–1580 (1978).
FinchJ.T. & A.Klug. Proc. Natl. Acad. Sci. USA 73: 1897–1901 (1976).
PospelovV.A., S.B.Svetlikova & V.I.Vorob'ev. Nucl. Acids Res. 4: 3267–3280 (1977).
PospelovV.A., S.B.Svetlikova & V.I.Vorob'ev. Molek. Biol. 11: 781–789 (1977).
ShawB.R., J.L.Corden, G.C.Sahasrabuddhe et al. Biochem. Biophys. Res. Commun. 61: 1193–1198 (1974).
PospelovV.A., S.B.Svetlikova & V.I.Vorob'ev.FEBS letters 74: 229–233 (1977).
MullerJ.J., G.Damaschun & G.Walter. Experimentalle Methoden der Molekulphysik 1: 11–30, Physikalische Gesellschaft der DDR, Reinhardsbrunn (1977).
DamaschunG., J.J.Muller & H.Bielka. Methods in Enzymology 59: 706–751 (1978).
FournetG.. In: Handbuch der Physik 32, 238–319, ed. by S.Flugge, Springer-Verlag, Berlin (1957).
KayushinaR., J.A.Rolbin & L.A.Feigin, Kristallografiya 19: 1161–1165 (1974).
MullerJ.J., G.Damaschun & H.Bielka. Eur. Journ. Biochem. 90: 547–553 (1978).
DubochetJ. & M.Noll. Science 202: 280–286 (1978).
SobellH.M., B.S.Reddy, K.H.Reddy, et al. Cold Spring Harbor Symp. Quant. Biol. 42: 87–102 (1978).
LuzzatiV., J.Witz & N.Nicolaieff. J. Mol. Biol. 3: 367–378 (1961).
GlatterO. J. Appl. Cryst. 12: 166–175 (1979).
Damaschun, G., H. Damaschun, H.-V. Purschel et al. Abh. Akdd. Wiss. DDR: 289–296 (1973).
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Damaschun, H., Damaschun, G., Pospelov, V.A. et al. X-ray small-angle scattering study of mononucleosomes and of the close packing of nucleosomes in polynucleosomes. Mol Biol Rep 6, 185–191 (1980). https://doi.org/10.1007/BF00775414
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DOI: https://doi.org/10.1007/BF00775414