Abstract
A double-stranded RNA-specific nuclease (ds R Nase) has been isolated and partially purified from human placenta by DEAE-cellulose and DNA-cellulose column chromatography. Denatured DNA-cellulose retained most of the single-stranded RNA-specific nuclease (ss R Nase) activity, whereas the ds R Nase came out in the void volume. N-ethylmaleimide at a concentration of 5 mM, selectively inhibited ds R Nase activity by 60% under the conditions in which the ss R Nase activity was inhibited to an extent of 7%. The ds R Nase was specifically inhibited byPenicillium chrysogenum viral ds RNA and by ethidium bromide. The partially purified ds R Nase showed requirements for Mg2+ whereas Mn2+ and NH sup+inf4 ions were inhibitory. The DEAE-enzyme cleaved32P-labelled 45S ribosomal precursor RNAs from Yoshida ascites sarcoma cells into species that had similar electrophoretic mobilities as the mature rRNAs.
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Kalyanaraman, S., Maran, A. & Shanmugam, G. Partial purification and characterization of a double-stranded RNA-specific nuclease from human placenta. Mol Biol Rep 9, 179–183 (1983). https://doi.org/10.1007/BF00775365
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DOI: https://doi.org/10.1007/BF00775365