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Perdeuterioacetylation with combined NMR and MS analysis as a method for determining the evolution of individual hydrolysis products during regioselective enzymatic hydrolysis of glucose pentaacetate

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Abstract

A new procedure is described that is based on NMR and MS analysis of the products of perdeuterioacetylation of the products of partial hydrolysis of β-D-glucose pentaacetate by the lipase fromAspergillus niger. This procedure eliminates the need for separation and estimation of each individual product and is of general applicability.

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Authors and Affiliations

  1. Department of Chemistry, University of Warwick, CV4 7AL, Coventry, UK

    David Chaplin, David H. G. Crout, David W. Hutchinson & Oliver W. Howarth

  2. Tate and Lyle Speciality Sweeteners, P.O. Box 68, RG6 2BX, Whiteknights, Reading, UK

    Riaz Khan

Authors
  1. David Chaplin
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  2. David H. G. Crout
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  3. David W. Hutchinson
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  4. Oliver W. Howarth
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  5. Riaz Khan
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Chaplin, D., Crout, D.H.G., Hutchinson, D.W. et al. Perdeuterioacetylation with combined NMR and MS analysis as a method for determining the evolution of individual hydrolysis products during regioselective enzymatic hydrolysis of glucose pentaacetate. Catal Lett 9, 71–83 (1991). https://doi.org/10.1007/BF00769084

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  • DOI: https://doi.org/10.1007/BF00769084

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