Abstract
Four chromium salts with different oxidation states were tested for their influence in causing chromosome aberrations and sister-chromatid exchange in Chinese hamster ovary cellsin vitro. Cell cultures were treated with CrO3, K2Cr2O7, CrCl2 and Cr(NO3)3.9H2O at concentrations of 10−7, 10−6, 10−5 and 10−4 M for the aberration assay, and 10−8, 10−7, 10−6 and 10−5 M for the sister-chromatid exchange assay. It was noticed that Cr (VI) compounds-CrO3 and K2Cr2O7-considerably enhanced the frequencies of aberrations and sister-chromatid exchanges compared to the control cultures. CrCl2 and Cr(NO3)3.9H2O−Cr (II) and Cr (III) respectively-caused a slight increase in sister-chromatid exchange rates, but the frequencies of aberrations were almost unchanged compared to the controls. These investigations indicate a definite link between the metals and changes produced in the mammalian chromosomes, reaffirming the evidence of carcinogenic potential of Cr (VI) observed by other investigators.
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Abbreviations
- BrdU:
-
5-bromo-2′-deoxyuridine
- CHO:
-
Chinese hamster ovary
- SCE:
-
sister-chromatid exchange
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Kochhar, T.S., Howard, W.R. Effect of different oxidation states of chromium in causing chromosome alterations in cultured CHO cells. Cytotechnology 11, 183–188 (1993). https://doi.org/10.1007/BF00749868
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DOI: https://doi.org/10.1007/BF00749868