Abstract
A murine hybridoma cell line producing a monoclonal antibody against penicillin-G-amidase and a murine transfectoma cell line secreting a monovalent chimeric human/mouse Fab-antibody fragment were cultivated in three different media (serum-containing, low protein serum-free, and iron-rich protein-free) in flask cultures, stirred reactors and a fixed bed reactor. In static batch cultures in flasks both cell lines showed similar good growth in all three media.
In suspension in a stirred reactor, the hybridoma cell line could be cultivated satisfactory only in serum-containing medium. In low protein serum-free medium, Pluronic F68 had to be added to protect the hybridoma cells against shear stress. But even with this supplement only batch, not chemostat mode was possible. In iron-rich protein-free medium the hybridoma cells grew also in continuous chemostat mode, but the stability of the culture was low. The transfectoma cell line did not grow in stirred reactors in any of the three media.
Good results with both cell lines were obtained in fixed bed experiments, where the cells were immobilized in macroporous Siran®-carriers. The media, which were optimized in flask cultures, could be used without any further adaptation in the fixed bed reactor. Immobilization improved the stability and reliability of cultures of non-adherent animal cells in serum-free media tremendously compared to suspension cultures in stirred reactors. The volume-specific glucose uptake rate, an, indicator of the activity of the immobilized cells, was similar in all three media. Deviations in the metabolism of immobilized and suspended cells seem to be mainly due to low oxygen concentrations within the macroporous carriers, where the cells are supplied with oxygen only by diffusion.
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Abbreviations
- c:
-
substrate or product concentration mmol l−1
- c0 :
-
substrate or product concentration in the feed mmol l−1
- cGlc :
-
glucose concentration mmol l−1
- cGln :
-
glutamine concentration mmol l−1
- cAmm :
-
ammonia concentration mmol l−1
- cLac :
-
lactate concentration mmol l−1
- cFAB :
-
concentration of Fab# 10 antibody fragment μg l−1
- cMAb :
-
monoclonal antibody concentration mg l−1
- D:
-
dilution rate d−1
- q:
-
cell-specific substrate uptake or metabolite production rate mmol cell−1 h−1
- qGlc :
-
cell-specific glucose uptake rate mmol cell−1 h−1
- qGln :
-
cell-specific glutamine uptake rate mmol cell−1 h−1
- qMAb :
-
cell-specific MAb production rate mg cell−1 h−1
- q* :
-
volume-specific substrate uptake or metabolite production rate mmol l−1 h−1
- q*FB:
-
volume-specific substrate uptake or metabolite production rate related to the fixed bed volume mmol lFB −1 h−1
- q*FB,Glc:
-
volume-specific glucose uptake rate related to the fixed bed volume mmol lFB −1 h−1
- q*FB,Gln:
-
volume-specific glutamine uptake rate related to the fixed volume mmol lFB −1 h−1
- q*FB,MAb:
-
volume-specific MAb production rate related to the fixed volume mg lFB −1 h−1
- q*FB,02:
-
volume-specific oxygen uptake rate related to the fixed bed volume mmol lFB −1 h−1
- t:
-
time h
- U:
-
superficial flow velocity mm s−1
- V:
-
medium volume in the conditioning vessel of the fixed bed reactor l
- VFB :
-
volume of the fixed bed l
- xv :
-
viable cell concentration cells ml−1
- yAmm,Gln :
-
yield of Ammonia from glutamine
- yLac,Glc :
-
yield of lactate from glucose
- μ:
-
specific growth rate h−1
- μd :
-
specific death rate h−1
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Lüdemann, I., Pörtner, R., Schaefer, C. et al. Improvement of the culture stability of non-anchorage-dependent animal cells grown in serum-free media through immobilization. Cytotechnology 19, 111–124 (1995). https://doi.org/10.1007/BF00749766
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DOI: https://doi.org/10.1007/BF00749766