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Stage-specific expression of fuco-neolacto- (Lewis X) and ganglio-series neutral glycosphingolipids during brain development: characterization of Lewis X and related glycosphingolipids in bovine, human and rat brain

  • Papers Dedicated To Dr Sen-Itiroh Hakomori
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Abstract

We have purified and characterized a bovine brain pentaglycosylceramide as Lewis X and identified it in human and rat brain using anti-Lewis X (anti-SSEA 1) monoclonal antibody. Neutral glycosphingolipid expression in developing rat brain has been examined by digoxigenin immunostaining and TLC-immunostaining using anti-SSEA 1 and anti-GgOse4Cer (GA1) monoclonal antibodies. Five transient Lewis X-series bands were identified in brain at embryonic day 15 that disappear by postnatal day 5 (one disappears at embryonic day 18). Gangliotetraosylceramide (GA1) first appears at embryonic day 21 and increases in concentration with age until postnatal day 21. In addition, we have purified another minor brain neutral glycosphingolipid and tentatively identified it as a Lewis X-series glycolipid by gas chromatography-mass spectrometry analysis followed by TLC-immunostaining with anti-SSEA 1 antibody.

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Abbreviations

Cer:

Ceramide, GlcCer, Glcβ1-1Cer

LacCer:

Galβ1-4GlcCer

CTH:

Galα1-4LacCer

nLcOse4Cer:

Galβ1-4GlcNAcβ1-3LacCer

nLcOse5Cer:

Galα1-3nLcOse4Cer

GgOse4Cer:

Galβ1-3GalNAcβ1-4LacCer

DPA:

diphenylamine-aniline-phosphoric acid

SSEA:

stage-specific embryonic antigen

NGSL:

neutral glycosphingolipid

TLC:

thin-layer tomography

HPTLC:

high performance thin-layer chromatography

GA1:

gangliotetraosyleramide

SAT-2:

sialytransferase-2

GalNAcT-1:

galactosaminyltransferase-1

DIG-IS:

digoxigenin-immunostaining

PMAAS:

partially methylated alditol acetates

DCE:

dichloroethane

TLC-IS:

TLC-immunostaining

(Lex):

Lewis X

NK:

murine natural killer

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Authors and Affiliations

  1. Department of Neurology, Medical University of South Carolina, 171 Ashley Avenue, 29425-2232, Charleston, SC, USA

    Somsankar Dasgupta, Edward L. Hogan & Samuel S. Spicer

  2. Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 171 Ashley Avenue, 29425-2232, Charleston, SC, USA

    Samuel S. Spicer

Authors
  1. Somsankar Dasgupta
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  2. Edward L. Hogan
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  3. Samuel S. Spicer
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Dasgupta, S., Hogan, E.L. & Spicer, S.S. Stage-specific expression of fuco-neolacto- (Lewis X) and ganglio-series neutral glycosphingolipids during brain development: characterization of Lewis X and related glycosphingolipids in bovine, human and rat brain. Glycoconjugate J 13, 367–375 (1996). https://doi.org/10.1007/BF00731469

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  • DOI: https://doi.org/10.1007/BF00731469

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