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A 28-kDa cerebral neuropeptide fromManduca sexta: Relationship to the insect prothoracicotropic hormone

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Summary

  1. 1.

    A 28-kDa peptide from the brain of the tobacco hornworm,Manduca sexta, was purifiedvia HPLC. The peptide copurified with the insect neurohormone, prothoracicotropic hormone (PTTH), through two HPLC columns.

  2. 2.

    Immunocyctochemistry using polyclonal antibodies against the 28-kDa peptide revealed that the peptide was produced in the same protocerebral neurons that produce PTTH. Western blot analysis demonstrated that the 28-kDa peptide and big PTTH are different molecules.

  3. 3.

    A PTTHin vitro bioassay indicated that despite having chromatographic properties similar to those of big PTTH and being produced by the same neurons, the 28-kDa peptide did not have PTTH activity.

  4. 4.

    Amino acid sequence analysis yielded a 27 N-terminal amino acid sequence that had no similarity with known peptides.

  5. 5.

    Immunocytochemical studies revealed that the 28-kDa peptide is present as early as 30% embryonic development and is absent by adult eclosion. This is in contrast to big PTTH, which is expressed throughout theManduca life cycle.

  6. 6.

    These data suggest that the 28-kDa peptide is another secretory phenotype of the lateral neurosecretory cell group III (L-NSC III) which may have functions distinct from those for big PTTH or may act synergistically with big PTTH.

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Authors and Affiliations

  1. Department of Biology, CB 3280, Coker Hall 010A, University of North Carolina at Chapel Hill, Chapel Hill, 27599-3280, North Carolina, USA

    Rosemary S. Gray, David P. Muehleisen, Eva J. Katahira & Walter E. Bollenbacher

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  1. Rosemary S. Gray
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  2. David P. Muehleisen
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  3. Eva J. Katahira
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  4. Walter E. Bollenbacher
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Gray, R.S., Muehleisen, D.P., Katahira, E.J. et al. A 28-kDa cerebral neuropeptide fromManduca sexta: Relationship to the insect prothoracicotropic hormone. Cell Mol Neurobiol 13, 39–58 (1993). https://doi.org/10.1007/BF00712988

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