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Cytokeratin polypeptides expression in different epithelial elements of human salivary glands

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Summary

Immunofluorescent labeling of human salivary glands was carried out with a battery of monoclonal antibodies reactive with specific cytokeratin polypeptides. All the epithelial elements of the glands were positively labelled by a broad-spectrum cytokeratin antibody (KG 8.13) and by antibody Ks 18.18, which reacts with cytokeratin No. 18 exclusively. Labelling of frozen sections with antibody KM 4.62, which is reactive with the 40 Kd (No. 19) cytokeratin, was confined to the ductal system and apparently absent from the acini. Antibody KA-1, reactive with polypeptides 4, 5 and 6 stained both the myoepithelial cells and the basal cells of the large ducts. Antibody KS 8.58, however, reacted with the basal cells exclusively. It is thus proposed that the combined use of the various monoclonal antibodies may provide a most useful probe in studies on epithelial cell diversity in normal salivary glands as well as in pathological disorders of that gland.

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References

  • Anderton BH (1981) Intermediate filaments: A family of homologous structures. J Muscle Res Cell Motility 2:141–166

    Google Scholar 

  • Bannasch P, Zerban H, Schmid E, Franke WW (1980) Liver tumors distinguished by immunofluorescence microscopy with antibodies to proteins of intermediate sized filaments. Proc Natl Acad Sci USA 77:4948–4952

    Google Scholar 

  • Battifora H, Sun TT, Bahu RM, Rau S (1980) The use of antikeratin antiserum as a diagnostic tool: Thymoma versus lymphoma. Hum Pathol 11:635–641

    Google Scholar 

  • Brandtzaeg P (1973) Conjugates of immunoglobulin G with different fluorophores. I. Characterization by anionic exchange chromatography. Scand J Immunol 2:273–290

    Google Scholar 

  • Caselitz J, Löning T (1981) Specific demonstration of actin and keratin filaments in pleomorphic adenomas by means of immunoelectron microscopy. Virchows Arch [Pathol Anat] 393:153–158

    Google Scholar 

  • Caselitz J, Osborn M, Seifert G, Weber K (1981a) Intermediate sized filament proteins (prekeratin, vimentin, desmin) in normal parotid gland and parotid gland tumors. Virchows Arch [Pathol Anat] 393:273–286

    Google Scholar 

  • Caselitz J, Löning T, Staquet MJ, Seifert G, Thivolet J (1981 b) Immunocytochemical demonstration of filamentous structures in the parotid gland. J Cancer Res Clin Oncol 100:59–68

    Google Scholar 

  • Cutler LS, Chaudhry AP (1973) Differentiation of the myoepithelial cells of the rat submandibular gland in vivo and in vitro in an ultra structural study. J Morphol 140:343–354

    Google Scholar 

  • Czernobilsky B, Moll R, Levy R, Franke WW (1985) Co-expression of cytokeratin and vimentin filaments in mesothelial, granulosa and rete ovarii cells of human ovary. Eur J Cell Biol 37:175–190

    Google Scholar 

  • Dardick I, Van Nostrand AWP, Jeans MTD, Rippstein P, Edward V (1983) Pleomorphic adenoma: Ultrastructural organization of “epithelial” regions. Hum Pathol 14:780–797

    Google Scholar 

  • Dardick I, Daya D, Hardie J, Van Nostrand AWP (1984) Mucoepidermoid carcinoma: Ultrastructural and histogenetic aspects. J Oral Pathol 13:342–358

    Google Scholar 

  • Dardick I, Jeans MTD, Sinnott NM, Wittkuhor JF, Kahu HJ, Baumal R (1985) Salivary gland components involved in the formation of squamous metaplasia. Am J Pathol 119:33–43

    Google Scholar 

  • Debus E, Weber K, Osborn M (1982) Monoclonal cytokeratin antibodies that distinguish simple from stratified squamous epithelia: Characterization of human tissues. EMBO J 1:1641–1647

    Google Scholar 

  • Drenckhahn D, Groschel-Stewart U, Unsickler K (1977) Immunofluorescence microscopic demonstration of myosin and actin in salivary glands and exocrine pancreas of rat. Cell Tissue Res 183:273–279

    Google Scholar 

  • Eversole LR (1971) Histogenetic classification of salivary tumors. Arch Pathol 92:433–443

    Google Scholar 

  • Franke WW, Schmid E, Freudenstein C, Appelhans B, Osborn M, Weber K, Ullmann TW (1980) Intermediate sized filaments of the prekeratin type in myoepithelial cells. J Cell Biol 84:633–654

    Google Scholar 

  • Franke WW, Schmid E, Osborn M, Weber K (1978) Different intermediate sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci USA 75:5034–5088

    Google Scholar 

  • Franke WW, Schmid E, Freudenstein C, Appelhans B, Osborn M, Weber K, Keenan TW (1980) Intermediate-sized filaments of the prekeratin type in myoepithelial cells. J Cell Biol 84:633–654

    Google Scholar 

  • Franke WW, Schiller DL, Moll R, Winter S, Schmid E, Engelbrecht I, Denk H, Krepler R, Platzer B (1981) Diversity of cytokeratins. J Mol Biol 153:933–959

    Google Scholar 

  • Gabbiani G, Kapanci X, Barazzone P, Franke WW (1981) Immunochemical identification of intermediate sized filaments in human neoplastic cells: a diagnostic aid for the surgical pathologist. Am J Pathol 104:206–216

    Google Scholar 

  • Geiger B, Singer SJ (1979) Participation of α-actinin in the capping of membrane components. Cell 16:213–222

    Google Scholar 

  • Gigi-Leitner O, Geiger B (1986) Antigenic interrelationship between 40 KD cytokeratin polypeptide and desmoplakins. Cell Motil Cytoskel (in press)

  • Gigi O, Geiger B, Eshhar Z, Moll R, Schmid E, Winter S, Schiller DL, Franke WW (1982) Detection of a cytokeratin determinant common to diverse epithelial cells by a broadly cross-reacting monoclonal antibody. EMBO J 1:1429–1437

    Google Scholar 

  • Gigi-Leitner O, Geiger B, Levy R, Czernobilsky B (1986) Cytokeratin expression in squamous metaplasia of human uterine cervix. Differentiation 31:191–205

    Google Scholar 

  • Gusterson BA, Lucas RB, Ormerod MG (1982) Distribution of epithelial membrane antigen in benign and malignant lesions of salivary glands. Virchows Arch [Pathol Anat] 397:227–233

    Google Scholar 

  • Huszar M, Gigi-Leitner O, Moll R, Franke WW, Geiger B (1986) Polypeptide specific monoclonal cytokeratin antibodies in the differential diagnosis of squamous carcinomas and adenocarcinomas. Differentiation 31:141–153

    Google Scholar 

  • Krepler R, Denk H, Artlieb U, Moll R (1982) Immunocytochemistry of intermediate filament proteins present in pleomorphic adenomas of the human parotid gland. Characterization of different cell types in the same tumor. Differentiation 21:191–199

    Google Scholar 

  • Krikos GA (1978) “Salvary glands” in: Orban's Oral Histology and Embryology. Edited by Harry Sicker CV Mosby pp. 266–300

  • Lazarides E (1980) Intermediate filaments as mechanical integrators of cellular space. Nature 283:249–256

    Google Scholar 

  • Miettinen M, Lehto VP, Badley RA, Virtanen I (1982) Expression of intermediate filaments in soft tissue sarcomas. Int J Cancer 30:541–546

    Google Scholar 

  • Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalog of human cytokeratins. Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24

    Google Scholar 

  • Nagle RB, Bocker W, Davis JR, Heid HW, Kaufmann M, Lucas DO, Jarasch ED (1986) Characterization of breast carcinomas by two monoclonal antibodies distinguishing myoepithelial from luminal epithelial cells. J Histochem Cytochem 34:869–882

    Google Scholar 

  • Osborn M, Geisler N, Shaw G, Sharp G, Weber K (1982) Intermediate filaments. Cold Spring Harbor Symp Quant Biol 46:413–429

    Google Scholar 

  • Osborn M, Weber K (1982) Intermediate filaments: cell-type specific markers in differentiation and pathology. Cell 31:303–306

    Google Scholar 

  • Pinkstaff CA (1980) The cytology of salivary glands. Int Rev Cytol 63:140–261

    Google Scholar 

  • Quinlan RA, Schiller DL, Hatzfeld M, Achtastatter T, Moll R, Jorcano JL, Magin TM, Franke WW (1986) Patterns of expression and organization of cytokeratin intermediate filaments. In: Wang E, Fischman D, Liem RKH, Sun T-T (eds). Intermediate filaments. NY Acad Sci 55:282–306

    Google Scholar 

  • Ramaekers F, Huysmans A, Moesker O, Kant A, Jap P, Herman C, Vooijs P (1983) Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors use in surgical pathology. Lab Invest 49:353–359

    Google Scholar 

  • Ramaekers F, Puts J, Kank A, Moesker O, Jap P, Vooies G (1981) Use of antibodies to intermediate filaments in characterization of human tumors. Cold Spring Harbor Syrup Quant Biol 46:331–339

    Google Scholar 

  • Regezi JA, Batsakis JG (1977) Histogenesis of salivary gland neoplasms. Otolaryngol Clin North Am 10:297–307

    Google Scholar 

  • Schiller DL, Franke WW, Geiger B (1982) A subfamily of relatively large and basic cytokeratin polypeptides as defined by peptide mapping is represented by one or several polypeptide in epithelial cell. EMBO J 1:761–769

    Google Scholar 

  • Shackleford JM, Schneyer LH (1971) Ultrastructural aspects of the main excretory duct of rat submandibular gland. Anat Rec 169:679–696

    Google Scholar 

  • Sun T-T, Shih CH, Green H (1979) Keratin cytoskeletons in epithelial cells of internal organs. Proc Natl Acad Sci USA 76:2813–2817

    Google Scholar 

  • Sun T-T, Tseng SOG, Huang AJ-W, Cooper D, Schermer A, Lynch MH, Weiss R, Eichner R (1986) Monoclonal antibody studies of mammalian epithelial keratins. A review. In: Wang E, Fischman P, Liem R, Sun T-T (eds) Intermediate Filaments. Ann NY Acad Sci 455:307–329

    Google Scholar 

  • Tamarin A, Sreenby LM (1965) The rat submaxillary salivary gland. A correlative study by light and electron-microscopy. J Morphol 117:295–352

    Google Scholar 

  • Thackray AC, Lucas RB (1974) In: Tumors of the Major Salivary Glands. Atlas of tumor pathology. Second series Fascicle 10. Washington Armed Forces Institute of Pathology

  • Towbin H, Stachelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyachylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:(9) 4350–4354

    Google Scholar 

  • Vogel AM, Gown AM (1984) Monoclonal antibodies to intermediate filament proteins use in diagnostic surgical pathology. In: Shay J (ed) Cell and Muscle Motility. Plenum Publishing Corp, pp 397–402

  • Welsh RA, Meyer TA (1968) Mixed tumors of human salivary glands. Arch Pathol 85:433–447

    Google Scholar 

  • Wilkie TF (1970) The surgical treatment of drooling. Plast Reconstit Surg 45:549–554

    Google Scholar 

  • Young JA, Van Lennep EW (1978) In: The Morphology of Salivary Gland. Academic press (Lond, NY and San Francisco)

    Google Scholar 

  • Wulf E, Deboden A, Bantz FA, Faulstich H, Wieland TH (1979) Fluorescent phallotoxin, a tool for the visualization of cellular actin. Proc Natl Acad Sci USA 76:4498–4502

    Google Scholar 

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Authors and Affiliations

  1. Tel Aviv University School of Dental Medicine, Ramat Aviv, Israel

    Selly Geiger

  2. Department of Otolaryngology, Kaplan Hospital, Rehovot

    Gabriel Marshak

  3. Department of Chemical Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel

    Benjamin Geiger, Orith Leitner & Gabriel Marshak

  4. Hadassah Medical School, Hebrew University, Jerusalem, Israel

    Gabriel Marshak

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  1. Selly Geiger
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  2. Benjamin Geiger
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  3. Orith Leitner
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  4. Gabriel Marshak
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Geiger, S., Geiger, B., Leitner, O. et al. Cytokeratin polypeptides expression in different epithelial elements of human salivary glands. Vichows Archiv A Pathol Anat 410, 403–414 (1987). https://doi.org/10.1007/BF00712760

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